The role of flushing dental water lines for the removal of microbial contaminants.

Public Health Reports
Eugene W RiceDennis J Lye

Abstract

This study was designed to determine the role of flushing dental water lines for the removal of heterotrophic plate count bacteria, Legionella spp., and free-living protozoa. Forty dental offices were surveyed in the study. An initial sample and a sample taken after three minutes of flushing were obtained from the air/water syringe at each location. All samples were quantitatively analyzed for heterotrophic bacteria using three bacteriological procedures. The samples were analyzed for the presence of Legionella spp. using cultural, immunological, and molecular procedures and for the occurrence of free-living protozoa using a killed bacteria plate procedure. The flushing process reduced the level of heterotrophic plate count bacteria by 1.1 to 1.5 log10 CFU/ml. Compliance with recommendations for bacterial levels varied depending on the methodology employed in the analysis. The flushing process did not reduce the occurrence of Legionella spp. or free-living protozoa. The results support recent U.S. Centers for Disease Control and Prevention recommendations that the process of flushing dental water lines cannot be relied upon as a sole means of reliably improving the quality of water used in dental treatment.

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Citations

Jul 28, 2019·International Journal of Environmental Research and Public Health·Anna Maria SpagnoloMaria Luisa Cristina
Oct 19, 2011·Future Microbiology·Mary J O'DonnellDavid C Coleman
Apr 5, 2020·International Journal of Environmental Research and Public Health·Orlando J Castellano RealpeJacobus H de Waard
Feb 4, 2009·Journal of Applied Microbiology·D C ColemanR J Russell
Feb 20, 2020·The Saudi Dental Journal·Manal M AlkhulaifiThekra Binshoail
Jun 27, 2021·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Milad MalekshoarBahareh Javanshir

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