PMID: 8970990Dec 1, 1996Paper

The role of Gag in human immunodeficiency virus type 1 virion morphogenesis and early steps of the viral life cycle

Journal of Virology
A S ReicinS P Goff

Abstract

The phenotypes of a series of mutant human immunodeficiency virus type 1 proviruses with linker insertion and deletion mutations within the gag coding region were characterized. These mutants, with mutations in the matrix, capsid, and p2 coding regions, produced replication-defective virion particles with defects in the early steps of the viral life cycle. To investigate this phenotype further, the abilities of mutant virion particles to enter T cells, initiate and complete reverse transcription, and transport the newly transcribed proviral DNA were investigated. Only 4 of 10 of the mutants appeared to make wild-type levels of viral DNA. Biochemical analyses of the mutants revealed the middle region of CA as being important in determining virion particle density and sedimentation in velocity gradients. This region also appears to be critical in determining the morphology of mature virion particles by electron microscopy. Particles with aberrant morphology were uninfectious, and only those mutants which displayed cone-shaped cores were capable of carrying out the early steps of the viral life cycle. Thus, the normal morphology of human immunodeficiency virus type 1 appears to be critical to infectivity.

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Citations

Dec 9, 1997·AIDS Research and Human Retroviruses·K S ChangS C Twu
Jul 9, 1999·Reviews in Medical Virology·I M Jones, Y Morikawa
Dec 10, 1999·Journal of Virology·L J ParentR C Craven
Dec 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chun TangMichael F Summers
Feb 12, 1998·The Journal of Experimental Medicine·M DorschS P Goff
Apr 15, 2003·Journal of Virology·Uta K von SchwedlerWesley I Sundquist
Jun 23, 2004·Nature Structural & Molecular Biology·Jason LanmanPeter E Prevelige
Apr 14, 2005·Journal of Virology·Cagan GurerJeremy Luban
May 23, 2003·The Journal of Biological Chemistry·Marta del AlamoMauricio G Mateu
May 18, 2007·Clinical Pharmacology and Therapeutics·C W HendrixR Wahl
Sep 15, 2007·Journal of Virology·Marcy R AuerbachIla R Singh
Jun 19, 2009·Expert Opinion on Therapeutic Targets·Catherine S AdamsonEric O Freed
Nov 9, 2002·Transfusion·Asa OhagenAris Lazo
Jan 18, 2006·Journal of Virology·William FuWei-Shau Hu
Nov 10, 2006·Journal of Virology·Jared L SpidelJohn W Wills
Aug 30, 2020·Viruses·Alex B Kleinpeter, Eric O Freed
Sep 13, 2020·Viruses·Jennifer L Elliott, Sebla B Kutluay
Nov 9, 2000·Journal of Virology·A Ohagen, D Gabuzda

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