The role of gap junctions and mechanical loading on mineral formation in a collagen-I scaffold seeded with osteoprogenitor cells

Tissue Engineering. Part a
Swathi DamarajuNeil A Duncan

Abstract

Fracture nonunions represent one of many large bone defects where current treatment strategies fall short in restoring both form and function of the injured tissue. In this case, the use of a tissue-engineered scaffold for promoting bone healing offers an accessible and easy-to-manipulate environment for studying bone formation processes in vitro. We have previously shown that mechanical prestimulation using confined compression of differentiating osteoblasts results in an increase in mineralization formed in a 3D collagen-I scaffold. This study builds on this knowledge by evaluating the short and long-term effects of blocking gap junction-mediated intercellular communication among osteogenic cells on their effectiveness to mineralize collagen-I scaffolds in vitro, and in the presence and absence of mechanical stimulation. In this study, confined compression was applied in conjunction with octanol (a general communication blocker) or 18-α-glycerrhetinic acid (AGA, a specific gap junction blocker) using a modified FlexCell plate to collagen-I scaffolds seeded with murine embryonic stem cells stimulated toward osteoblast differentiation using beta-glycerol phosphate. The activity, presence, and expression of osteoblast cadherin, ...Continue Reading

References

May 1, 1978·The Journal of Bone and Joint Surgery. British Volume·B McKibbin
May 1, 1991·Molecular Reproduction and Development·B DaleK Elder
Jan 1, 1983·Metabolic Bone Disease & Related Research·A R VillanuevaA M Parfitt
Aug 7, 1998·Molecular Biology of the Cell·F LecandaR Civitelli
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B ChengJ X Jiang
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anna WredenbergNils-Göran Larsson
Jan 30, 2004·Exercise and Sport Sciences Reviews·Ted S GrossSundar Srinivasan
Feb 24, 2004·Medical & Biological Engineering & Computing·M MullenderJ Klein-Nulend
Feb 8, 2005·Biochemical and Biophysical Research Communications·Joseph P Stains, Roberto Civitelli
Mar 17, 2006·Medicine and Science in Sports and Exercise·Brent A BakerRobert G Cutlip
Jul 25, 2006·European Journal of Cell Biology·Andrew D WaggettJames R Ralphs
Aug 4, 2006·American Journal of Physiology. Cell Physiology·A F TaylorH J Donahue
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Jean Xin JiangSirisha Burra
Dec 1, 2006·Clinical & Experimental Metastasis·Jennifer HalpernGinger E Holt
Jan 27, 2007·Journal of Applied Physiology·Sundar SrinivasanTed S Gross
Feb 16, 2007·Journal of Cellular Physiology·Damian C GenetosHenry J Donahue
Apr 3, 2007·The Journal of Hand Surgery·Clemens DumontKlaus Michael Stürmer
Apr 20, 2007·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·C DumontK M Stürmer
Jul 24, 2007·Biomaterials·Ranieri CanceddaMaddalena Mastrogiacomo
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Nov 22, 2008·Joint, Bone, Spine : Revue Du Rhumatisme·Odile GabayChristelle Sanchez
Jan 13, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·In-Hwan SongJames E Dennis
Oct 13, 2009·Journal of Biomechanics·Jan-Hung ChenCraig A Simmons
Apr 15, 2011·Injury·Josh E Schroeder, Rami Mosheiff
Oct 4, 2011·Biochimica Et Biophysica Acta·Nidhi BatraJean X Jiang
Oct 29, 2011·Arthritis and Rheumatism·Christelle SanchezYves E Henrotin
Dec 14, 2011·Connective Tissue Research·Mi-Na KangJung-Keug Park
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nidhi BatraJean X Jiang
Jun 13, 2012·Journal of Tissue Engineering and Regenerative Medicine·John C IgweSyam P Nukavarapu
Jun 19, 2012·Acta Biomaterialia·Ana Marina FerreiraGianluca Ciardelli
Sep 15, 2012·Computer Methods in Biomechanics and Biomedical Engineering·Jane Desrochers, Neil A Duncan

❮ Previous
Next ❯

Citations

Dec 7, 2016·Journal of Cellular Biochemistry·Dawei WangFusheng Dong
Mar 21, 2019·American Journal of Physiology. Cell Physiology·Michael J McClureZvi Schwartz
May 8, 2020·Connective Tissue Research·Roxanne Fournier, Rene E Harrison
Sep 25, 2019·Journal of Clinical Medicine·Galit Katarivas LevyAthina E Markaki
Feb 20, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yinfeng Lyu, Chenchen Feng

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism
NIH ImageJ
FlexCell
Zen

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.