The role of Glu196 in the environment around the substrate binding site of leucine aminopeptidase from Streptomyces griseus

FEBS Letters
Jiro ArimaTadashi Hatanaka


To investigate the role of Glu196 of leucine aminopeptidase from Streptomyces griseus (SGAP) in SGAP activation by calcium and substrate specificity, we constructed E196X SGAP by saturation mutagenesis. Most mutations led to the abrogation of SGAP activation by calcium, and substitution with Lys led to a marked increase in activity toward Asp-p-nitroanilide (pNA) and a decrease in that toward Lys-pNA. A similar result was obtained from the investigation using non-calcium-activated enzyme from Streptomyces septatus (SSAP). These results indicate that Glu196 of SGAP is associated with the environment around the substrate binding site besides its role in SGAP activation by calcium.


Jan 1, 1976·Methods in Enzymology·J M Prescott, S H Wilkes
Dec 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·G J SanderinkG Siest
Apr 1, 1993·Journal of Molecular and Cellular Cardiology·M J CurtisP D Ridley
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Taylor
Feb 7, 1997·Journal of Molecular Biology·H M GreenblattGil Shoham
Apr 20, 2000·Acta Crystallographica. Section D, Biological Crystallography·R GilboaGil Shoham
Dec 12, 2002·Chemical Reviews·W Todd Lowther, Brian W Matthews
May 13, 2004·The Journal of Biological Chemistry·Krzysztof P Bzymek, Richard C Holz
Jul 29, 2004·FEBS Letters·Yifat Fundoiano-HershcovitzYuval Shoham

Related Concepts

Bacterial Proteins
Enzyme Activation
Methoxyleucine Aminopeptidase
Streptomyces griseus
Substrate Specificity
Mutagenesis Process
Point Mutation

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