The role of GRHL2 and epigenetic remodeling in epithelial-mesenchymal plasticity in ovarian cancer cells

Communications Biology
Vin Yee ChungRuby Yun-Ju Huang

Abstract

Cancer cells exhibit phenotypic plasticity during epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) involving intermediate states. To study genome-wide epigenetic remodeling associated with EMT plasticity, we integrate the analyses of DNA methylation, ChIP-sequencing of five histone marks (H3K4me1, H3K4me3, H3K27Ac, H3K27me3 and H3K9me3) and transcriptome profiling performed on ovarian cancer cells with different epithelial/mesenchymal states and on a knockdown model of EMT suppressor Grainyhead-like 2 (GRHL2). We have identified differentially methylated CpG sites associated with EMT, found at promoters of epithelial genes and GRHL2 binding sites. GRHL2 knockdown results in CpG methylation gain and nucleosomal remodeling (reduction in permissive marks H3K4me3 and H3K27ac; elevated repressive mark H3K27me3), resembling the changes observed across progressive EMT states. Epigenetic-modifying agents such as 5-azacitidine, GSK126 and mocetinostat further reveal cell state-dependent plasticity upon GRHL2 overexpression. Overall, we demonstrate that epithelial genes are subject to epigenetic control during intermediate phases of EMT/MET involving GRHL2.

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Citations

Sep 29, 2019·Journal of Clinical Medicine·Mohit Kumar Jolly, Toni Celià-Terrassa
Apr 9, 2020·Frontiers in Molecular Biosciences·Stanislav DrápelaKarel Souček
Dec 16, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Yuan LiangJiang Du
Dec 31, 2020·Journal of Clinical Medicine·Satwik PasaniMohit Kumar Jolly

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Methods Mentioned

BETA
immunoprecipitation
ChIP-seq
PCR
ChIP
fluorescence-activated cell sorting
RNA-seq
Infrared Imaging

Software Mentioned

bedtools
ENCODE Irreproducible
phantompeakqualtools
Novoalign
BinReg
SAM
EBseq
ROC
Homer
FastQC

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