The Role of GSK3β in the Regulation of IL-10 and IL-12 Production Induced by Lipopolysaccharide in PK-15 Cells

DNA and Cell Biology
Yan WangLinjie Wang

Abstract

Glycogen synthase kinase-3β (GSK3β) plays a fundamental role in the inflammatory response by controlling the balance between pro- and anti-inflammatory cytokine production induced by lipopolysaccharide (LPS). However, the inflammatory roles of different porcine GSK3β isoforms in response to LPS remain unknown. In this study, the activity of porcine GSK3β was inhibited in response to LPS stimulation through increased serine 9 phosphorylation of GSK3β, which increased the production of interleukin (IL)-10 and IL-12 in PK-15 cells. However, treatment with an inhibitor of GSK3β, lithium chloride, significantly increased the production of IL-10 and IL-12 compared with the untreated control and significantly decreased the production of IL-10 and IL-12 compared with LPS stimulation. Moreover, all four glycogen synthase kinase-3 transcripts had positive roles in the production of IL-10 and IL-12 to varying degrees, suggesting their importance in the LPS-induced inflammatory response in pigs.

References

Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P S Klein, D A Melton
Mar 20, 2002·Cytokine & Growth Factor Reviews·Mario P Colombo, Giorgio Trinchieri
Sep 12, 2003·Trends in Pharmacological Sciences·Richard S Jope
Apr 23, 2004·Trends in Biochemical Sciences·Richard S Jope, Gail V W Johnson
May 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wendy NobleKaren Duff
Aug 17, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Guillermo I Perez-PerezAsalia Z Olivares
Apr 6, 2007·International Journal of Experimental Pathology·Toby Lawrence, Derek W Gilroy
Mar 28, 2008·Immunological Reviews·Magnus JohanssonLisa M Coussens
Oct 4, 2008·Neuropsychobiology·V L MisenerUNKNOWN International Consortium for Childhood-Onset Mood Disorders
Nov 11, 2008·The Journal of Biological Chemistry·Masaaki NakayamaToshiya Hirayama
Oct 20, 2009·Trends in Immunology·Eléonore BeurelRichard S Jope
Nov 26, 2009·The Journal of Allergy and Clinical Immunology·Scott P ComminsJohn W Steinke
Nov 24, 2011·Frontiers in Molecular Neuroscience·Oksana Kaidanovich-Beilin, James Robert Woodgett
Jun 14, 2012·Journal of Inflammation·Ricarda Cortés-VieyraVíctor M Baizabal-Aguirre
Jul 17, 2012·Neurochemistry International·Holly F Green, Yvonne M Nolan
Jan 30, 2013·Molecular and Cellular Biochemistry·Linjie WangYuanzhu Xiong

❮ Previous
Next ❯

Citations

Oct 31, 2018·Scientific Reports·Linjie WangYan Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis
transfection
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Alzheimer's Disease
Peter CrouchKatja Kanninen
© 2021 Meta ULC. All rights reserved