The role of hematogenous and intrinsic precursor cells in lymphocyte production in murine bone marrow and thymus

The American Journal of Anatomy
J L MejinoC Rosse

Abstract

It is well recognized that the bone marrow contains cells that can repopulate a depleted thymus as well as cells that can be induced to express phenotypic markers characteristic of T cells. It is not known, however, to what extent thymocytopoiesis in the normal thymus relies on immigrant, bone marrow-derived cells, nor whether some T cell precursors have entered the bone marrow from the circulation. We used the parabiotic system to test whether thymocytopoiesis relies on progenitors intrinsic to the thymus or on cells that enter the organ from the circulation. In the same system, we have also investigated whether Thy-1- bone marrow lymphocytes that respond to phytohemagglutinin (PHA) by proliferation and Thy-1 expression are produced by myelogenous or hematogenous progenitors. Syngeneic CBA/HT6 and CBA/CaJ mice were joined in parabiotic union at 4-6 weeks of age. Cross circulation between the two partners was verified by the equilibration of Evans' blue dye injected into one partner and by the equilibration of PHA-responsive T cells in the spleen of the parabionts. Chromosome spreads were prepared from the PHA-stimulated T cell-depleted bone marrow and from spontaneously proliferating thymocytes as well as from thymocytes stimu...Continue Reading

References

Feb 20, 2008·Current Topics in Developmental Biology·Deepa Manwani, James J Bieker

Citations

Jan 1, 1978·Immunological Reviews·O Stutman
Feb 28, 1975·Annals of the New York Academy of Sciences·M P ScheidE A Boyse
Mar 1, 1978·The Journal of Experimental Medicine·R M ZinkernagelJ Klein
Dec 1, 1979·The Journal of Experimental Medicine·H O BesedovskyE Sorkin
Jan 1, 1977·Contemporary Topics in Immunobiology·O Stutman
Sep 1, 1976·European Journal of Immunology·F LoorG E Roelants
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Mar 24, 1973·British Medical Journal·S F O'Beirn
Aug 15, 1980·Cellular Immunology·V Brankovan, C Rosse
Feb 29, 1964·Nature·J E HARRISE P EVANS

Related Concepts

Precursor Cells, T-Lymphoid
T-Lymphocyte
Bone Marrow Lymphocytes (Lab Test)
Colony-Forming Units, Hematopoietic
Cross Circulation
Spleen Colony-forming Unit
Spleen
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Neoplasm of Uncertain or Unknown Behavior of Thymus
Murine

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