The role of heme oxygenase-1 in the proliferation and odontoblastic differentiation of human dental pulp cells

Journal of Endodontics
Sun-Ju KimEun-Cheol Kim

Abstract

It was recently reported that heme oxygenase-1 (HO-1) activity is related to stem cell differentiation; however, the involvement of HO-1 in pulp cell growth and differentiation has not been well explored. The purpose of this study was to investigate the role of HO-1 in the growth and differentiation of human dental pulp cells (HDPCs). We evaluated cell growth by MTT assay, mineralization by alizarin red staining, and differentiation marker mRNA expression by reverse transcriptase polymerase chain reaction. HO-1 induction by cobaltic protoporphyrin IX (CoPP) in HDPCs increased cell growth and mineralization and up-regulated the messenger RNA expression of such odontoblastic markers as alkaline phosphatase, osteopontin, bone sialoprotein, dentin matrix protein-1, and dentin sialophosphoprotein. Carbon monoxide scavenger, iron chelator, HO-1 inhibitor, and HO-1 small interfering RNA (siRNA) attenuated HDPC growth and differentiation. CoPP treatment results in dental pulp cell proliferation and odontoblast differentiation that appears partly mediated by HO-1. Our results suggest that odontoblastic differentiation and growth are positively regulated by HO-1 induction and negatively regulated by HO-1 inhibition. Thus, pharmacologic H...Continue Reading

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Citations

Sep 24, 2013·Antioxidants & Redox Signaling·Magdalena KozakowskaAlicja Jozkowicz
Nov 14, 2013·Stem Cells and Development·Young-Man LeeEun-Cheol Kim
Oct 14, 2011·Clinical Science·Anna Grochot-PrzeczekAlicja Jozkowicz
Jan 18, 2011·Journal of Endodontics·Chia-Yung LinMin-Huey Chen
Oct 2, 2012·Archives of Oral Biology·Hiroyo KarubeMasato Matsuoka
Dec 27, 2018·International Journal of Molecular Sciences·Huiju Lee, Yoon Kyung Choi
Sep 17, 2013·International Endodontic Journal·D S KimE C Kim
Dec 5, 2019·Journal of Applied Oral Science : Revista FOB·Nadyne Saab MessiasLarissa Moreira Spinola de Castro-Raucci
Jan 20, 2021·ACS Biomaterials Science & Engineering·Ben WangXiaolei Zhang

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