The role of hemocytes, serine protease inhibitors and pathogen-associated patterns in prophenoloxidase activation in the desert locust, Schistocerca gregaria

Peptides
Vanessa FranssensJozef Vanden Broeck

Abstract

The prophenoloxidase-activating system is an important component of the innate immune response of insects, involved in wound healing and melanotic encapsulation. In this paper we show that in the desert locust, Schistocerca gregaria, hemocytes, challenged with microbial elicitors, are indispensable for the limited proteolytic activation of prophenoloxidase (proPO) in plasma. In addition, we assessed the influence of serine protease inhibitors on the induction of PO-activity in plasma. While soybean Bowman-Birk inhibitor (SBBI) inhibited the PO activation by laminarin-treated hemocytes, the endogenous pacifastin-related inhibitors, SGPI-1 (S. gregaria pacifastin-related inhibitor-1) and SGPI-2 did not affect the PO-activity under similar conditions. On the other hand, real-time PCR analysis revealed that the transcripts, encoding SGPI-1-3, were more abundant in the fat body of immune challenged animals, as compared to control animals.

References

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Citations

Jan 7, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Giancarlo Lopez-MartinezDavid L Denlinger
May 14, 2009·BMC Evolutionary Biology·Bert BreugelmansJozef Vanden Broeck
Jan 15, 2014·Developmental and Comparative Immunology·Yan-Ran ZhaoJin-Xing Wang
Oct 29, 2013·Annual Review of Entomology·Xianhui Wang, Le Kang
May 13, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Charles Le PabicNoussithé Koueta
Apr 13, 2010·Peptides·Renato de MarcoAparecida S Tanaka
Apr 15, 2009·Insect Biochemistry and Molecular Biology·Bert BreugelmansJozef Vanden Broeck
Jun 26, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Congjing FengJianfeng Lu
Feb 20, 2013·Entomologia Experimentalis Et Applicata·Marta L Del CampoAndré Kessler
Jul 22, 2014·Archives of Insect Biochemistry and Physiology·Agnieszka Zdybicka-BarabasMałgorzata Cytryńska
Jun 21, 2016·General and Comparative Endocrinology·Tewodros Firdissa DuressaRoger Huybrechts
Sep 21, 2013·The Journal of Biological Chemistry·Rolando Perdomo-MoralesMarlene Porto-Verdecia

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