PMID: 12770004May 29, 2003Paper

The role of hemolymph proline as a nitrogen sink during blood meal digestion by the mosquito Aedes aegypti

Journal of Insect Physiology
D A GoldstrohmMichael A Wells

Abstract

Mosquitoes utilize the amino acids derived from blood meal protein to produce egg proteins. But the amino acids can also be used to produce egg lipid or can be oxidized for energy production. These latter two processes result in the release of nitrogen as toxic ammonia. Therefore, amino acids must be processed in such a way that amino acid nitrogen can be incorporated into non-toxic waste products. Proline is the predominant amino acid in the hemolymph of the adult female mosquito Aedes aegypti. After feeding on albumin meal, hemolymph proline levels increased five-fold over unfed levels, reached maximal levels in the first hours after feeding and remained high through oviposition. Hemolymph proline levels increased as the concentration of protein in the meal increased. When starved of sugar for 24 h prior to feeding on an albumin meal, hemolymph proline levels increased four-fold over the proline levels of non-starved mosquitoes. Proline levels after feeding on a protein deficient in essential amino acids, pike parvalbumin, increased to twice the levels of albumin fed mosquitoes. Based on these observations, we propose that mosquitoes utilize proline as a temporary nitrogen sink to store ammonia arising from deamination of blo...Continue Reading

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Citations

Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Michael A Singer
Apr 5, 2005·Insect Biochemistry and Molecular Biology·Patricia Y ScaraffiaMichael A Wells
Jun 14, 2003·Journal of Insect Physiology·P Y Scaraffia, M A Wells
Jan 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patricia Y ScaraffiaRoger L Miesfeld
Feb 27, 2010·BMC Developmental Biology·Wagner VitalCarlos Logullo
Aug 19, 2014·Parasites & Vectors·Virginia Belloni, Patricia Y Scaraffia
Nov 22, 2011·Journal of Insect Physiology·Dirk WeihrauchMichael J O'Donnell
Mar 9, 2010·Journal of Insect Physiology·Patricia Y ScaraffiaRoger L Miesfeld
Aug 27, 2014·Molecular Microbiology·Gerald D BaldridgeAnn M Fallon
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Nov 9, 2016·Scientific Reports·Jan PernerPetr Kopáček
Sep 13, 2007·Journal of the American Mosquito Control Association·Joanne Rampersad, David Ammons
Jul 20, 2014·Applied and Environmental Microbiology·Veronika MichalkovaSerap Aksoy
Aug 23, 2018·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Renato MartinsCarlos Logullo
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Sep 1, 2021·Metabolomics : Official Journal of the Metabolomic Society·Jhenifer Nascimento da SilvaCarlos Logullo
Aug 31, 2021·Frontiers in Cell and Developmental Biology·Eduardo J PatriarcaGabriella Minchiotti

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