The role of hepatic antioxidant capacity and hepatobiliary transporter in liver injury induced by isopsoralen in zebrafish larvae

Human & Experimental Toxicology
Y ZhangK Liu

Abstract

Isopsoralen is the main component of the Chinese medicine psoralen, which has antitumour activity and can be used for the treatment of osteoporosis. However, the mechanism behind its hepatotoxicity has not yet been elucidated. In this study, the hepatotoxicity of isopsoralen was investigated using zebrafish. Isopsoralen treatment groups of 25, 50 and 100 μM were established. The mortality, liver morphology changes, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver histopathology and mRNA levels of liver injury-related genes in zebrafish larvae were measured. The results showed that isopsoralen resulted in the development of malformed zebrafish, dose-dependent increases in ALT and AST, decreased liver fluorescence and weakened fluorescence intensity. Histopathological examination showed that high-dose isopsoralen caused a large number of vacuolated structures in the larvae liver. The polymerase chain reaction results showed a significant decrease in the mRNA levels of genes related to antioxidant capacity ( lfabp, gstp2 and sod1) and drug transport ( mdr1, mrp1 and mrp2), indicating that isopsoralen significantly inhibited liver antioxidant capacity and drug efflux capacity in zebrafish larvae....Continue Reading

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Citations

Jul 20, 2019·Journal of Applied Toxicology : JAT·Jingting HuoLuyong Zhang
Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoxue WuQing Chen
Jun 19, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rafael Xavier MartinsDavi Farias
Aug 13, 2021·Drug and Chemical Toxicology·Ruiying YuanJianqing Yu

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
PCR

Software Mentioned

Pro Plus
Image

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