Abstract
The objective of the study was to examine the effect of exogenous hydrogen sulde donor; sodium hydrosulfide (NaHS), on thefree radical generation, cNOS uncoupling in the myocardium, and diastolicfunction in old rats. To evaluate diastolic function of the heart, we used pressure-volume (PV) conductance catheter system (Millar Instruments, USA). It was shown that H2S levels in. the isolated mitochondria and whole heart homogenates obtained from old age rats were significantly lower comparing with adult animals. The markers of combined oxidative and nitrosative stress (the rate of 0 ₂⁰ H generation, pools of H₂0₂, diene conjugates, malondialdehyde, uric acid, the activity of iNOS, nitrate reductase, and NO pools) were increased in the old hearts in line with cNOS uncoupling. Such changes in NOS coupling resulted in the loss of diastolic relaxation (decrease of the rate of relaxation of the left ventricle (dp/dtmin) by 33%, 3-times increase of the end-diastolic pressure, 1.5-time increase of the time constant of left ventricular relaxation (Tau g) and 2-time increase of the end-diastolic stiffness). It has been found that NaHS inhibits oxidative and nitrosative stress, restores cNOS coupling and constitutive de novo synthesis of ni...Continue Reading
References
May 1, 1978·Analytical Biochemistry·M Mihara, M Uchiyama
Oct 7, 1991·FEBS Letters·M SalterS Moncada
Nov 1, 1982·Canadian Journal of Physiology and Pharmacology·J M McCord, R S Roy
May 15, 1984·European Journal of Biochemistry·M Huwiler, H Kohler
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Aug 1, 1996·Shock·C Szabó
Mar 30, 2001·The Journal of Biological Chemistry·U S KayyaliP M Hassoun
Apr 16, 2003·Traffic·William J BrownAnne Doody
Dec 6, 2003·Journal of Lipid Research·Grace Y SunAgnes Simonyi
Apr 12, 2005·Cardiovascular Research·Ulrich SchmidtRoger J Hajjar
Jul 20, 2005·Current Vascular Pharmacology·Zoltan UngváriPál Pacher
Feb 21, 2006·Cancer Research·Corinne E GriguerG Yancey Gillespie
Aug 1, 2007·Free Radical Biology & Medicine·Nathan S Bryan, Matthew B Grisham
Mar 19, 2008·Journal of Neurochemistry·Phullara B ShelatGrace Y Sun
Sep 6, 2008·Nature Protocols·Pál PacherDavid A Kass
Mar 3, 2009·Biochimica Et Biophysica Acta·Frank B Jensen
Dec 20, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Kieren G HollingsworthGuy A Macgowan
Jul 24, 2012·Free Radical Biology & Medicine·Yixuan ZhangAn L Moens
Oct 2, 2012·Biochimica Et Biophysica Acta·Michelle L AspJoseph M Metzger
Feb 9, 2013·Circulation·Kazuhisa KondoDavid J Lefer
Jul 4, 2013·Free Radical Biology & Medicine·Nathan D RoeJun Ren
Aug 8, 2013·Aging Cell·Gilles GouspillouPhilippe Diolez
Oct 8, 2013·BMC Geriatrics·Melanie J Sullivan-Gunn, Paul A Lewandowski
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Adrienne L KingDavid J Lefer
Feb 15, 2014·Circulation Research·David J Polhemus, David J Lefer