PMID: 9555975Apr 29, 1998Paper

The role of IL-10 in iNOS and cytokine mRNA expression during in vitro differentiation of bovine mononuclear phagocytes

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
W L GoffC R Wyatt

Abstract

In the study reported here, we used RT-PCR with primers specific for interleukin-1 (IL-1), IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), and nitric oxide synthase (iNOS) to assess the cytokine mRNA expression associated with bovine blood monocytes during their differentiation to macrophages cultured on plastic (1 week). In addition, we used RT-PCR to assess the contribution of gammadelta T cells as a source of interferon-gamma (IFN-gamma), the induction signal for iNOS. Further, we evaluated cytocentrifuge preparations from the cultures for the production of IL-10 using specific antibody. We previously demonstrated that iNOS can be induced in cultured bovine monocytes in response to IFN-gamma and TNF-alpha but lose this capability in a short period of time. However, we demonstrate here that iNOS induction from monocytes cultured with IFN-gamma secreting gammadelta T cells is prolonged, suggesting that this source of IFN-gamma primes the monocytes before exogenous stimulation. Based on mRNA expression, placement of monocytes in culture resulted in activation, followed by quiescence. By 6 days in culture, the iNOS message was reduced below the basal level. In addition, the TNF-alpha message was substantially reduced, and ...Continue Reading

References

Jan 1, 1992·Progress in Growth Factor Research·D RennickG Holland
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·L DroogmansA Burny
Mar 1, 1986·Cellular Immunology·M J SteinbeckM L Kaeberle
Oct 1, 1988·Veterinary Immunology and Immunopathology·J A EllisW I Morrison
Sep 26, 1988·Nucleic Acids Research·S R LeongP W Gray
May 16, 1988·Biochemical and Biophysical Research Communications·B BeutlerD Crawford
Jan 28, 1994·Biochemical and Biophysical Research Communications·H AdlerT W Jungi
May 1, 1993·Journal of Leukocyte Biology·M S RutherfordL B Schook
Jul 23, 1996·Annals of the New York Academy of Sciences·W C JohnsonC R Wyatt
Feb 1, 1997·Cellular Immunology·R S GieniR H DeKruyff

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Citations

Jun 5, 2004·Developmental and Comparative Immunology·Carol G Chitko-McKownWilliam W Laegreid
Nov 26, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·W L TrigonaD M Estes
Jul 21, 1998·Annals of the New York Academy of Sciences·W L GoffC W Cluff
Oct 17, 2002·Annals of the New York Academy of Sciences·W L GoffW C Davis
Jul 22, 2009·Antimicrobial Agents and Chemotherapy·O Nicolas SchwintGlen A Scoles
May 5, 2009·Veterinary Immunology and Immunopathology·M S KhalifehJ R Stabel
Mar 6, 2007·Veterinary Immunology and Immunopathology·Stephanie WiddisonTracey J Coffey

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