The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

Ocular Immunology and Inflammation
Choul Yong ParkChung-Gyu Park

Abstract

To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-gamma stimulation. RPEs expressed IDO and class II MHC molecules under IFN-gamma stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-gamma prestimulation. This phenomenon was enhanced by IFN-gamma stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. The expression of IDO may in part explain RPE-mediated immune suppression effects.

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Citations

Jul 22, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhenyang ZhaoJiyang Cai
Feb 20, 2019·International Journal of Molecular Sciences·Katrin Spekker-BoskerSilvia Kathrin Eller

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