The role of insulin, glucagon, dexamethasone, and leptin in the regulation of ketogenesis and glycogen storage in primary cultures of porcine hepatocytes prepared from 60 kg pigs

Domestic Animal Endocrinology
I Fernández-FígaresThomas J Caperna

Abstract

A study was conducted to elucidate hormonal control of ketogenesis and glycogen deposition in primary cultures of porcine hepatocytes. Hepatocytes were isolated from pigs (54-68 kg) by collagenase perfusion and seeded into collagen-coated T-25 flasks. Monolayers were established in medium containing fetal bovine serum for 1 day and switched to a serum-free medium for the remainder of the culture period. Hepatocytes were maintained in DMEM/M199 containing 1% DMSO, dexamethasone (10(-6) or 10(-7) M), linoleic acid (3.4 x 10(-5) M), and carnitine (10(-3) M) for 3 days. On the first day of serum-free culture, insulin was added at 1 or 100 ng/ml and glucagon was added at 0, 1, or 100 ng/ml. Recombinant human leptin (200 ng/ml) was added during the final 24 h; medium and all cells were harvested on the third day. Concentrations of acetoacetate and beta-hydroxybutyrate (ketone bodies) in media and glycogen deposition in the cellular compartment were determined. Ketogenesis was highly stimulated by glucagon (1 and 100 ng/ml) and inhibited by insulin. In contrast, glycogen deposition was stimulated by insulin and attenuated by glucagon; high insulin was also associated with a reduction in the ketone body ratio (acetoacetate:beta-hydroxy...Continue Reading

References

Jun 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C VonsD Franco
Jul 1, 1991·The Journal of Nutrition·M T CoffeyE E Jones
Sep 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y HaranoY Shigeta
Dec 1, 1985·Journal of Animal Science·T J CapernaN C Steele
Jun 1, 1983·Analytical Biochemistry·T R Downs, W W Wilfinger
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraA Ichihara
Jul 1, 1982·The Journal of Nutrition·M J MüllerH J Seitz
Jul 1, 1993·The British Journal of Nutrition·A J LepineD M Whitehead
Aug 1, 1995·Molecular and Cellular Biochemistry·S Pugazhenthi, R L Khandelwal
Apr 1, 1997·Journal of Dairy Science·C Cadórniga-ValiñoS J Bertics
Nov 5, 1997·The Journal of Biological Chemistry·L RossettiJ Wang
Mar 14, 1998·Journal of Animal Science·T G RamsayC Morrison
May 5, 1998·Intensive Care Medicine·A de JaegerM Lambert
Nov 13, 1998·The Journal of Biological Chemistry·L LiuL Rossetti
Nov 14, 1998·Circulation Research·A BouloumiéR Busse
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M NemeczM Roden
Dec 1, 1999·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·R B CeddiaR Curi
Feb 19, 2000·Biochemical and Biophysical Research Communications·I A LeclercqG R Robertson
Feb 7, 2001·The Journal of Biological Chemistry·A Z ZhaoK E Bornfeldt
Apr 28, 2000·Cell Transplantation·P G GregoryS J Sullivan
Jul 27, 2000·Molecular Reproduction and Development·Z T Ruiz-CortésB D Murphy

❮ Previous
Next ❯

Citations

Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Ryan R WillardThomas J Caperna
Mar 23, 2012·Animal : an International Journal of Animal Bioscience·J A Conde-AguileraI Fernández-Fígares
Jun 21, 2007·Cloning and Stem Cells·Shirou MatsumotoFumio Endo
Jul 16, 2014·Animal : an International Journal of Animal Bioscience·L González-ValeroI Fernández-Fígares
Nov 3, 2010·Nutrition·Stephen CunnaneStanley I Rapoport
Aug 21, 2018·World Journal of Gastroenterology : WJG·Yun HuangYi Gao
Sep 30, 2020·Molecular Nutrition & Food Research·Yuting ChengWalee Chamulitrat

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Priya RamanMichael E Spurlock
International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity
R B CeddiaR Curi
© 2022 Meta ULC. All rights reserved