The Role of Intercalated Cell Nedd4-2 in BP Regulation, Ion Transport, and Transporter Expression

Journal of the American Society of Nephrology : JASN
Masayoshi NanamiS M Wall

Abstract

BackgroundNedd4-2 is an E3 ubiquitin-protein ligase that associates with transport proteins, causing their ubiquitylation, and then internalization and degradation. Previous research has suggested a correlation between Nedd4-2 and BP. In this study, we explored the effect of intercalated cell (IC) Nedd4-2 gene ablation on IC transporter abundance and function and on BP.Methods We generated IC Nedd4-2 knockout mice using Cre-lox technology and produced global pendrin/Nedd4-2 null mice by breeding global Nedd4-2 null (Nedd4-2-/- ) mice with global pendrin null (Slc26a4-/- ) mice. Mice ate a diet with 1%-4% NaCl; BP was measured by tail cuff and radiotelemetry. We measured transepithelial transport of Cl- and total CO2 and transepithelial voltage in cortical collecting ducts perfused in vitro Transporter abundance was detected with immunoblots, immunohistochemistry, and immunogold cytochemistry.Results IC Nedd4-2 gene ablation markedly increased electroneutral Cl-/HCO3- exchange in the cortical collecting duct, although benzamil-, thiazide-, and bafilomycin-sensitive ion flux changed very little. IC Nedd4-2 gene ablation did not increase the abundance of type B IC transporters, such as AE4 (Slc4a9), H+-ATPase, barttin, or the Na+-...Continue Reading

References

Nov 1, 1990·Pflügers Archiv : European journal of physiology·E SchlatterJ A Schafer
Dec 1, 1988·The Journal of Clinical Investigation·D BrownS Gluck
Oct 22, 1987·The New England Journal of Medicine·T W KurtzR C Morris
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·W GüntherT J Jentsch
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·G H KimM A Knepper
Mar 13, 1999·The Journal of Clinical Investigation·H AbrielO Staub
Oct 8, 1999·The Journal of Clinical Investigation·S MasilamaniM A Knepper
Jan 8, 2000·Physiology & Behavior·A C CostaM T Davisson
Dec 26, 2000·The Journal of Biological Chemistry·M SchwakeT J Jentsch
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·I E RoyauxE D Green
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Susan M WallJill W Verlander
Apr 10, 2003·American Journal of Physiology. Renal Physiology·Anke DahlmannLawrence G Palmer
Oct 8, 2003·Current Protein & Peptide Science·Pierre M Viallet, Tuan Vo-Dinh
Oct 19, 2004·The Journal of Biological Chemistry·Deanne H HryciwPhilip Poronnik
Apr 9, 2005·American Journal of Physiology. Renal Physiology·Omar A ItaniChristie P Thomas
Jul 22, 2005·Nature·Alessandra Picollo, Michael Pusch
Sep 8, 2005·American Journal of Physiology. Renal Physiology·Young-Hee KimSusan M Wall
Mar 31, 2006·Journal of the American Society of Nephrology : JASN·Dominique Loffing-CueniJohannes Loffing
Apr 8, 2006·Physiological Reviews·Olivier Staub, Daniela Rotin
May 4, 2006·American Journal of Physiology. Renal Physiology·Jill W VerlanderSusan M Wall
Nov 2, 2006·American Journal of Physiology. Renal Physiology·Vladimír PechSusan M Wall
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Young Hee KimSusan M Wall
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Feb 23, 2008·Clinical and Experimental Hypertension : CHE·Hao WenXiaofeng Wang
Jun 6, 2008·American Journal of Physiology. Renal Physiology·Peijun P ShiBaoli Yang
Feb 18, 2010·Proceedings of the American Thoracic Society·Douglas C EatonLucky Jain
May 28, 2010·American Journal of Physiology. Renal Physiology·Nandita S RaikwarChristie P Thomas
Oct 26, 2010·Pflügers Archiv : European journal of physiology·Daniela Rotin, Olivier Staub
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Toshihiro KimuraDaniela Rotin
Apr 21, 2011·Nature Communications·Natasha A BoaseSharad Kumar
May 20, 2011·American Journal of Physiology. Renal Physiology·Naohiro NomuraShinichi Uchida
Aug 20, 2011·Journal of the American Society of Nephrology : JASN·Juan Pablo ArroyoOlivier Staub

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Citations

Sep 13, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Rawad LashhabEmmanuelle Cordat
Apr 30, 2020·Physiological Reviews·Susan M WallCesar A Romero
Apr 15, 2020·The Journal of Clinical Investigation·Maria Agustina BattistoneSylvie Breton
Apr 16, 2019·American Journal of Hypertension·Shigeru Shibata
Aug 1, 2020·International Journal of Molecular Sciences·Osamu YamazakiShigeru Shibata
Oct 11, 2020·Journal of Cellular Physiology·Yuan HuangJingfeng Tang

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