The role of interface framework residues in determining antibody V(H)/V(L) interaction strength and antigen-binding affinity

The FEBS Journal
Kenji MasudaHiroshi Ueda

Abstract

While many antibodies with strong antigen-binding affinity have stable variable regions with a strong antibody heavy chain variable region fragment (V(H))/antibody light chain variable region fragment (V(L)) interaction, the anti-lysozyme IgG HyHEL-10 has a fairly strong affinity, yet a very weak V(H)/V(L) interaction strength, in the absence of antigen. To investigate the possible relationship between antigen-binding affinity and V(H)/V(L) interaction strength, a novel phage display system that can switch two display modes was employed. We focused on the two framework region 2 regions of the HyHEL-10 V(H) and V(L), facing each other at the domain interface, and a combinatorial library was made in which each framework region 2 residue was mixed with that of D1.3, which has a far stronger V(H)/V(L) interaction. The phagemid library, encoding V(H) gene 7 and V(L) amber codon gene 9, was used to transform TG-1 (sup+), and the phages displaying functional variable regions were selected. The selected phages were then used to infect a nonsuppressing strain, and the culture supernatant containing V(H)-displaying phages and soluble V(L) fragment was used to evaluate the V(H)/V(L) interaction strength. The results clearly showed the exi...Continue Reading

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Citations

Mar 23, 2011·Biochemistry. Biokhimii︠a︡·E P AltshulerA G Katrukha
Jul 28, 2012·Journal of Molecular Biology·Everardo Remi Rodríguez-RodríguezLidia Riaño-Umbarila
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jan 30, 2008·Protein Science : a Publication of the Protein Society·Takeshi NakanishiIzumi Kumagai
Sep 29, 2009·Protein Science : a Publication of the Protein Society·K Reed Clark, Scott T R Walsh
Jan 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Patrick KoenigGermaine Fuh
Sep 30, 2020·Journal of Cancer Research and Clinical Oncology·Shuyu HuangAndrea van Elsas
Jul 12, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Sylvia DietrichGordana Wozniak-Knopp
Mar 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Bharat MadanBrandon J DeKosky
Nov 21, 2009·Analytical Chemistry·Keiko GionHideyuki Inui
Oct 6, 2007·Journal of Molecular Biology·Juan Pablo AciernoAna Cauerhff

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