The Role of Intracellular Receptor NODs for Cytokine Production by Macrophages Infected with Mycobacterium leprae.

Immune Network
Tae Jin Kang, Gue-Tae Chae

Abstract

The nucleotide-oligomerization domain (NOD) proteins are members of the NOD-like receptor (NLR) family, which are intracellular and cytoplasmic receptors. We analyzed the role of NODs for cytokine production by macrophages infected with intracellular pathogen M. leprae, the causative agent of leprosy. Production of pro-inflammatory cytokines such as IL-1β and TNF-α was inhibited in the presence of cytochalasin D, an agent blocking phagocytosis, suggesting that intracellular signaling was, partially, required for macrophage activation to M. leprae infection. Next, we investigated the role of NOD1 and NOD2 proteins on NF-κB activation and cytokine expression. Treatment with M. leprae significantly increased NF-κB activation and expression of TNF-α and IL-1β in NOD1- and NOD2-transfected cells. Interestingly, their activation and expression were inhibited by cytochalasin D, suggesting that stimulation of NOD proteins may be associated with the enhancement of cytokine production in host to M. leprae.

References

Jan 7, 2003·The Journal of Biological Chemistry·Naohiro InoharaGabriel Nuñez
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Jul 8, 2009·The Journal of Experimental Medicine·François CoulombeMarcel A Behr

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Citations

Jan 19, 2013·Cornea·Sung J KimJaeku Kang
Sep 19, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Wanyan Deng, Jianping Xie
Jan 6, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Young-Su Yi
Apr 13, 2018·Frontiers in Immunology·Roberta Olmo PinheiroEuzenir Nunes Sarno

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Methods Mentioned

BETA
ELISA
transfection

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