The role of LINC00094/miR-224-5p (miR-497-5p)/Endophilin-1 axis in Memantine mediated protective effects on blood-brain barrier in AD microenvironment

Journal of Cellular and Molecular Medicine
Lu ZhuXiuli Shang

Abstract

The dysfunction of the blood-brain barrier (BBB) is one of the main pathological features of Alzheimer's disease (AD). Memantine (MEM), an N-methyl-d-aspartate (NMDA) receptor antagonist, has been reported that been used widely for AD therapy. This study was performed to demonstrate the role of the MEM in regulating BBB permeability in AD microenvironment as well as its possible mechanisms. The present study showed that LINC00094 was dramatically increased in Abeta1-42 -incubated microvascular endothelial cells (ECs) of BBB model in vitro. Besides, it was decreased in MEM-incubated ECs. Silencing LINC00094 significantly decreased BBB permeability, meanwhile up-regulating the expression of ZO-1, occludin and claudin-5. Furthermore, silencing LINC00094 enhance the effect of MEM on decreasing BBB permeability in AD microenvironment. The analysis of the mechanism demonstrated that reduction of LINC00094 inhibited Endophilin-1 expression by up-regulating miR-224-4p/miR-497-5p, promoted the expression of ZO-1, occludin and claudin-5, and ultimately alleviated BBB permeability in AD microenvironment. Taken together, the present study suggests that the MEM/LINC00094/miR-224-5p (miR-497-5p)/Endophilin-1 axis plays a crucial role in the ...Continue Reading

References

Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·N RingstadP De Camilli
Jul 31, 2009·Journal of Neuroscience Research·George M AlleyDebomoy K Lahiri
Nov 12, 2009·Nature Reviews. Neuroscience·Stephen M EackerValina L Dawson
Aug 6, 2010·Brain Research Reviews·Filipa Lourenço CardosoMaria Alexandra Brito
Jan 5, 2011·Nature Reviews. Neuroscience·Lars M Ittner, Jürgen Götz
Aug 2, 2011·Cell·Leonardo SalmenaPier Paolo Pandolfi
Nov 4, 2011·Nature Reviews. Neuroscience·Berislav V Zlokovic
Oct 3, 2012·Trends in Cell Biology·Masashi Abe, Nancy M Bonini
Nov 29, 2012·The Journal of Clinical Investigation·Keita SodaShuta Ishibe
Sep 28, 2013·Journal of Alzheimer's Disease : JAD·Mihaela GheorghiuEugen Gheorghiu
Dec 7, 2013·Nature Medicine·Birgit ObermeierRichard M Ransohoff
Nov 23, 2015·Cell·Zhen ZhaoBerislav V Zlokovic
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Mimi BukeiratXuefang Ren
Mar 10, 2017·Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring·Cláudia Y SantosJessica Alber
Apr 14, 2017·Expert Opinion on Therapeutic Targets·Lian-Ju LiDong-Qing Ye
Apr 22, 2017·Journal of Molecular Neuroscience : MN·Fei WangTao Sun
May 31, 2017·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Marina BogushYuri Persidsky
Jun 7, 2017·Physical Biology·Panagiotis E TheodorakisOmar K Matar
Jul 7, 2017·Oxidative Medicine and Cellular Longevity·Juhyun SongOh Yoen Kim
Sep 18, 2017·Biochemical and Biophysical Research Communications·Kaori Takahashi-ItoTaisuke Tomita
Nov 21, 2017·Experimental Cell Research·Wenjing ChenChunyan Zhang
Nov 25, 2017·Journal of Alzheimer's Disease : JAD·Cátia D PereiraSandra Rebelo
Dec 14, 2017·International Journal of Molecular Sciences·Loredana LeggioNunzio Iraci

❮ Previous
Next ❯

Citations

Dec 20, 2020·International Journal of Molecular Sciences·Leticia Moreno-GarcíaRosario Osta
Feb 19, 2021·Ecotoxicology and Environmental Safety·Yan LiChunhui Ni
Aug 5, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yi XuYunfu Cui
Jun 8, 2021·Pharmacogenomics and Personalized Medicine·Dubravka Svob StracNela Pivac
Jul 27, 2021·International Journal of Clinical Practice·Omayma O AbdelaleemRania H Mahmoud

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
immunoprecipitation
RIP

Software Mentioned

Chem Imager 5500
GraphPad
miRanda
Targetscan
GraphPad Prism
FluorChem

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Related Papers

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Michelle A Erickson, William A Banks
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
K D MullenA J McCullough
© 2022 Meta ULC. All rights reserved