The role of lipase and alpha-amylase in the degradation of starch/poly(epsilon-caprolactone) fiber meshes and the osteogenic differentiation of cultured marrow stromal cells.

Tissue Engineering. Part a
Ana M MartinsA G Mikos

Abstract

The present work studies the influence of hydrolytic enzymes (alpha-amylase or lipase) on the degradation of fiber mesh scaffolds based on a blend of starch and poly(epsilon-caprolactone) (SPCL) and the osteogenic differentiation of osteogenic medium-expanded rat bone marrow stromal cells (MSCs) and subsequent formation of extracellular matrix on these scaffolds under static culture conditions. The biodegradation profile of SPCL fiber meshes was investigated using enzymes that are specifically responsible for the enzymatic hydrolysis of SPCL using concentrations similar to those found in human serum. These degradation studies were performed under static and dynamic conditions. After several degradation periods (3, 7, 14, 21, and 30 days), weight loss measurements and micro-computed tomography analysis (specifically porosity, interconnectivity, mean pore size, and fiber thickness) were performed. The SPCL scaffolds were seeded with rat MSCs and cultured for 8 and 16 days using complete osteogenic media with and without enzymes (alpha-amylase or lipase). Results indicate that culture medium supplemented with enzymes enhanced cell proliferation after 16 days of culture, whereas culture medium without enzymes did not. No calcium wa...Continue Reading

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Citations

Jul 30, 2009·Journal of Materials Science. Materials in Medicine·Iva PashkulevaRui L Reis
Jan 1, 2014·Tissue Engineering. Part a·Nicky de JongeCarlijn V C Bouten
Oct 16, 2015·Tissue Engineering. Part a·Marieke BrugmansAnita Driessen-Mol
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Nov 21, 2016·International Journal of Biological Macromolecules·Imran KhanRamakrishnan Ganesan

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Methods Mentioned

BETA
X-ray
infrared spectroscopy
DNA assay
confocal microscopy

Software Mentioned

ANT 3D creator
CT Analyser
SkyScan
NRecon

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