PMID: 6411988Jul 1, 1983Paper

The role of lipoproteins in steroidogenesis by human luteinized granulosa cells in culture

Journal of Steroid Biochemistry
R W TureckJ F Strauss

Abstract

Progesterone secretion by primary cultures of luteinized human granulosa cells was markedly reduced when the cells were incubated in lipoprotein-deficient medium. Addition of LDL, but not HDL3, to cells cultured in lipoprotein-deficient medium stimulated progestin secretion. The effects of LDL were dose-dependent and saturable (Km = 5.5 micrograms LDL protein/ml). LDL also stimulated [3H]-oleate incorporation into cellular sterol esters, with half maximal stimulation occurring at LDL concentrations of 10 micrograms protein/ml. The cultured cells bound and internalized [125I]-LDL in a dose dependent and saturable manner (Km = 5-10 micrograms LDL protein/ml). [125I]-LDL uptake was specific in that unlabeled LDL, but not unlabeled HDL3, competed with labeled LDL for uptake [125I]-HDL3 was also taken up by the cells, but by a lower affinity mechanism. We conclude that luteinized human granulosa cells utilize LDL-carried cholesterol for progestin synthesis, and that LDL is taken up via a specific, high affinity process.

References

Sep 1, 1972·The Journal of Clinical Endocrinology and Metabolism·G O DeVillaG Flickinger
Jan 1, 1981·Advances in Lipid Research·J F StraussT Tanaka
May 1, 1982·Endocrinology·M OhashiE R Simpson
May 1, 1981·The Journal of Clinical Endocrinology and Metabolism·B R CarrE R Simpson

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Citations

Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Lane K Christenson, Luigi Devoto

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