Abstract
The aim of the current study was to identify the long noncoding RNAs (lncRNAs) ANRIL function and molecular pathways underlying hepatocellular carcinoma progression. ANRIL knockdown with specific siRNA, and transfected into HepG2 cells according to the protocol of Lipofectamine 2000. Cell proliferation, apoptosis, migration and metastasis were assessed with MTT assay, flow cytometry and wound healing assay, respectively. Moreover, the expression level of ANRIL, apoptosis-related genes, and the Wnt pathway-associated genes were assessed by real time-PCR and Western blot assay. Knocking down of ANRIL led to alleviated cell growth and increased cell apoptosis of HepG2 cells through markedly increased expression levels of Bax and Bad. In contrast, dramatically diminished the expressions of anti-apoptotic factors including Bid and Bcl-2 in comparison to the scrambled control group (si-NC). Furthermore, ANRIL silencing resulted in an inactivated Wnt/β-catenin pathway by suppressing key genes associated with this pathway. Taken together, these findings imply new insights into the regulatory network of the Wnt pathway through lncRNA ANRIL that indicate ANRIL may be a therapeutic factor potential for hepatocellular carcinoma.
References
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Oct 20, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eric PasmantIvan Bièche
Jan 18, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Thanh-Tuan HuynhChi-Tai Yeh
Aug 5, 2014·DNA Repair·Bahman YousefiYasin Ahmadi
Nov 6, 2015·Nature Medicine·Maite Huarte
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
Jun 21, 2016·EBioMedicine·Chi Han Li, Yangchao Chen
Jan 1, 2015·Journal of Hematology & Oncology·Ming-de HuangYong-Qian Shu
Jun 27, 2017·Cancer Cell·Jittiporn ChaisaingmongkolUNKNOWN TIGER-LC Consortium
Jul 14, 2017·Cancer Research·Arunoday BhanSubhrangsu S Mandal
Nov 12, 2017·Journal of Cancer Research and Clinical Oncology·Ji MaHuifeng Yuan
Apr 10, 2018·Ageing Research Reviews·Maryam MajidiniaBahman Yousefi
Sep 11, 2018·OncoTargets and Therapy·Weiying DaiSong Jin
Sep 27, 2018·BMC Cancer·Deyu HuangHua Liu
Nov 21, 2018·Biotechnology Advances·Seyed Mohammad NabaviSamira Shirooie
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 1, 2019·International Journal of Biological Macromolecules·Feifei YuGuiqiu Zhao
Feb 18, 2019·EBioMedicine·Pasqualino de Antonellis
Apr 11, 2019·The New England Journal of Medicine·Augusto Villanueva
May 28, 2019·Molecular Therapy. Nucleic Acids·Zheng ChenZhiyu Xiao
Jun 21, 2019·Dose-response : a Publication of International Hormesis Society·Hongwen CaoChao Yu
Jul 14, 2019·Journal of Experimental & Clinical Cancer Research : CR·Ang ZhengFeng Jin
Oct 17, 2019·Biomolecules·Joshua M Adams, Hamed Jafar-Nejad