The role of mAKAPβ in the process of cardiomyocyte hypertrophy induced by angiotensin II

International Journal of Molecular Medicine
Huixin GuoYidong Wei

Abstract

Angiotensin II (AngII) is the central product of the renin-angiotensin system (RAS) and this octapeptide contributes to the pathophysiology of cardiac hypertrophy and remodeling. mAKAPβ is an A-kinase anchoring protein (AKAP) that has the function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. In this study, we aimed to investigate the role of mAKAPβ in AngII‑induced cardiomyocyte hypertrophy and the possible mechanisms involved. Cultured cardiomyocytes from neonatal rats were treated with AngII. Subsequently, the morphology of the cardiomyocytes was observed and the expression of mAKAPβ and cardiomyocyte hypertrophic markers was measured. mAKAPβ‑shRNA was constructed for RNA interference; the expression of mAKAPβ and hypertrophic markers, the cell surface area and the [3H]Leucine incorporation rate in the AngII‑treated rat cardiomyocytes were detected following RNA interference. Simultaneously, changes in the expression levels of phosphorylated extracellular signal-regulated kinase (p-ERK)2 in the cardiomyocytes were assessed. The cell size of the AngII-treated cardiaomyocytes was significantly larger than that of the untreated cardiomyocytes. ...Continue Reading

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Citations

May 5, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Sailaja Paruchuri, Charles K Thodeti
Feb 27, 2020·Circulation Research·Shiju YeGuang Liang
Jan 25, 2020·Molecular Medicine Reports·Huijun ChengYoujun Zhou
Jun 29, 2017·International Journal of Molecular Medicine·Jianrong XuHao Li

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Methods Mentioned

BETA
PCR
transfection
protein assay
fluorescence microscopy

Software Mentioned

NIH ImageJ
Oligo
SPSS

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