PMID: 2119810Aug 27, 1990Paper

The role of mammalian initiation factor eIF-4D and its hypusine modification in translation

Biochimica Et Biophysica Acta
J W HersheyJ Schnier

Abstract

Initiation factor eIF-4D functions late in the initiation pathway, apparently during formation of the first peptide bond. The factor is post-translationally modified at a specific lysine residue by reaction with spermidine and subsequent hydroxylation to form hypusine. A precursor form lacking hypusine is inactive in the assay for methionyl-puromycin synthesis, but activity is restored following in vitro modification to deoxyhypusine, thereby suggesting that the modification is essential for function. Since formylated methionyl-tRNA is less dependent on eIF-4D in the puromycin assay, we postulate that eIF-4D and its hypusine modification may stabilize charged Met-tRNA binding to the peptidyl transferase center of the 60S ribosomal subunit. Analysis of eIF-4D genes in yeast indicate that eIF-4D and its hypusine modification are essential for cell growth.

References

Jan 1, 1985·Annual Review of Biochemistry·K Moldave
Mar 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J W Hershey, R E Thach

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Citations

Aug 1, 1992·Antonie van Leeuwenhoek·P Linder
May 1, 1994·Molecular Biology Reports·H O VoormaH A Van Heugten
Sep 5, 2012·Amino Acids·Camila Arnaldo Olhê DiasSandro Roberto Valentini
Dec 1, 1992·Pharmacology & Therapeutics·A E Pegg, P P McCann
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·D BevecJ Hauber
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·N C Kyrpides, C R Woese
Jan 10, 2014·Wiley Interdisciplinary Reviews. RNA·Danuza RossiSandro Roberto Valentini
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
Nov 20, 2015·Journal of Immunology Research·Juan Santiago Salas-Benito, Mónica De Nova-Ocampo
Dec 14, 2001·Protein Engineering·A M FacchianoG Colonna
Jun 21, 2007·Amino Acids·C F Zanelli, S R Valentini
Jan 31, 2013·World Journal of Stem Cells·Gry H DihaziHassan Dihazi
Oct 19, 1995·Biochimica Et Biophysica Acta·M TerakuraS Otani

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