The role of MAP kinases in rapid gene induction after lesioning of the rat sciatic nerve

The European Journal of Neuroscience
H ZrouriF Courtin

Abstract

Lesion of the sciatic nerve caused a rapid activation of p38MAP kinase in the injured nerve adjacent to the site of transection. This activation was detectable 3 min after lesioning, increased during the next 15 min and remained high for several hours. Erk1/2 activation was also observed as early as 15 min after lesioning. Activation of these MAP kinases was seen in both the external sheaths and the endoneurium. The separation of the external sheaths from the endoneurium accelerated the p38MAP kinase activation. To evaluate whether the injury-activated MAP kinase cascades are implicated in the rapid gene induction observed after nerve lesion, experiments were performed with an ex vivo model. Segments of sciatic nerves were incubated in oxygenated Krebs-Ringer buffer. MAP kinases were activated at 15 min and remained active after 6 h. Induction of mRNA was also observed for nerve growth factor (NGF), interleukin 6 (IL-6), leukaemia inhibitory factor (LIF) and deiodinases of type 2 (D2) and type 3 (D3). Thus, the ex vivo model mimics events occurring in the animal after nerve section. Finally, nerve segments were incubated in the presence of specific inhibitors of Erk1/2 activation (U0126) and of p38MAP kinase activity (SB203580)...Continue Reading

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Citations

Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Françoise CourtinMichel Pierre
Jul 25, 2012·Thyroid : Official Journal of the American Thyroid Association·Mírian RomittiAna Luiza Maia
Jan 24, 2009·Science·Marc HammarlundMichael Bastiani
May 25, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David P YangHaesun A Kim
Nov 28, 2015·The European Journal of Neuroscience·Nguyen H TuSeiji Ito
Sep 14, 2010·Current Opinion in Neurobiology·Zhiping Wang, Yishi Jin
Aug 11, 2006·Journal of Neurochemistry·Shlomit Hanz, Mike Fainzilber
May 11, 2015·Experimental Neurology·André HeinenPatrick Küry
Jul 3, 2013·Stem Cells Translational Medicine·Haesun A KimDavid B Parkinson
Dec 12, 2013·Nature Reviews. Neuroscience·Ida Rishal, Mike Fainzilber
Mar 21, 2015·The Journal of Biological Chemistry·Andrei V ChernovVeronica I Shubayev
Apr 19, 2008·Endocrinology·Audrey LamirandFrançoise Courtin
Jun 11, 2017·Cellular and Molecular Life Sciences : CMLS·Nicolas Tricaud, Hwan Tae Park
Mar 7, 2017·Frontiers in Molecular Neuroscience·Angélique BoerboomRachelle Franzen

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