PMID: 11911089Mar 26, 2002Paper

The role of mass spectrometry in proteome studies

Biomolecular Engineering
T A Nyman

Abstract

Mass spectrometry (MS) is an important tool in modern protein chemistry. In proteome analyses the expression of hundreds or thousands of proteins can be monitored at the same time. First, complex protein mixtures are separated by two-dimensional gel electrophoresis (2-DE) and then individual proteins are identified by using MS followed by database searches. Recent developments in this field have made it possible to do automated, high-throughput protein identification that is needed in proteome analyses. MS can also be used to characterize post-translational modifications in proteins and to study protein complexes. This review will introduce the current MS methods used in proteome studies, and discuss their advantages and disadvantages. New instrumental MS developments are also presented that are useful in these analyses.

References

Nov 1, 1993·Analytical Biochemistry·J R YatesT Hunkapiller
Aug 31, 1993·Biochemical and Biophysical Research Communications·P JamesG Gonnet
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J HenzelC Watanabe
Jan 1, 1996·Analytical Chemistry·M Wilm, M Mann
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Mar 1, 1997·Electrophoresis·L Anderson, J Seilhamer
Apr 18, 1998·Nature Medicine·D Dickson
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
Jun 29, 1999·Analytical Chemistry·T A BrettellR Saferstein
Jul 15, 1999·Nature Biotechnology·A J LinkJ R Yates
Jul 29, 1999·Analytical Chemistry·M C Posewitz, P Tempst
Sep 3, 1999·Nature Biotechnology·E NordhoffK Kristiansen
Nov 24, 1999·Analytical Chemistry·P A BinzD F Hochstrasser
Feb 23, 2000·The Journal of Cell Biology·M P RoutB T Chait
Apr 29, 2000·Electrophoresis·G L CorthalsJ C Sanchez
May 18, 2000·Analytical Chemistry·A ShevchenkoK G Standing
Jun 14, 2000·The Journal of Biological Chemistry·H Vihinen, J Saarinen
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P GygiR Aebersold

❮ Previous
Next ❯

Citations

Nov 20, 2003·The Journal of Laboratory and Clinical Medicine·Ruben Bonilla-Guerrero, Gregory A Poland
Dec 6, 2003·Analytical and Bioanalytical Chemistry·A BossiP G Righetti
Jun 30, 2009·Journal of Proteomics·Angelo D'AlessandroLello Zolla
Dec 11, 2002·Expert Opinion on Therapeutic Targets·Christine H Cho, Mark E Nuttall
Mar 29, 2002·Journal of Mass Spectrometry : JMS
Feb 22, 2002·Journal of Mass Spectrometry : JMS
Sep 25, 2004·Mass Spectrometry Reviews·Heike Brötz-OesterheltHarald Labischinski
Aug 10, 2012·Current Protocols in Microbiology·Nadja Raatschen, Julia Elisabeth Bandow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Trends in Biotechnology
E T Maggio, K Ramnarayan
Genome Génome / Conseil National De Recherches Canada
Glyn Jenkins
© 2022 Meta ULC. All rights reserved