The role of matrix metalloproteinases in intestinal epithelial wound healing during normal and inflammatory states
Abstract
Healing of the epithelium is a key consideration in gastrointestinal surgery. Inflammation is one factor innate to patients with inflammatory bowel disease that poses a risk of delayed healing of the intestinal epithelium postoperatively. Epithelial wounding model was performed on rat intestinal epithelial cells grown under control and interferon gamma (IFN-γ)-, interleukin-1beta (IL-1β)-, and tumor necrosis factor-alpha (TNF-α)-stimulated conditions. Wounds were measured and percent healing was calculated at 0, 8 and 24 h. Western blot analysis was performed using matrix metalloproteinase (MMP)-7 primary antibody and semiquantitative densitometry was conducted. Wounds were 50.0% and 99.7% healed under control conditions at 8 and 24 h, respectively. IL-1β and IFN-γ delayed wound closure. MMP-7 increased by 2.3-fold at 8 h and 1.6-fold at 24 h during wound healing. Activated MMP-7 increased by 3- to 5-fold at 24 h. IL-1β stimulation increased levels of MMP-7 by 17% to 37% above the elevated expression due to healing alone. TNF-α up-regulated MMP-7 in non-wounded and wounded cells, and IFN-γ did not affect its expression. When MMP-7 activity was blocked, wound closure was delayed. MMP-7 significantly contributes to intestinal epi...Continue Reading
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