The Role of MCPIP1 in Ischemia/Reperfusion Injury-Induced HUVEC Migration and Apoptosis

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Tiebing ZhuJ Chao

Abstract

Monocyte chemotactic protein-induced protein 1 (MCPIP1) plays a crucial role in various cellular processes, including neurogenesis. However, the relationship between MCPIP1 and myocardial ischemia/reperfusion (I/R) injury remained illdefined. In this study, we explored whether the I/R-mediated up-regulation of MCPIP1 is critical in the modulation of both cell migration and apoptosis in human umbilical vein endothelial cells (HUVECs). Using Western blot analysis and quantitative real-time PCR, the protein expression and mRNA transcription, respectively, of MCPIP1 was detected in HUVECs. To investigate cell migration, an in vitro scratch assay and a nested matrix model were applied. I/R increased the expression of MCPIP1 via the activation of the mitogen-activated protein kinase (MAPK) and PI3K/Akt pathways. I/R increased migration and apoptosis of HUVECs, which were significantly inhibited by MCPIP1 siRNA. conclusion: These findings suggest that I/R-mediated up-regulation of MCPIP1 regulates migration and apoptosis in HUVECs. Understanding the regulation of MCPIP1 expression and function may aid in the development of an adjunct therapeutic strategy in the treatment of individuals with I/R injury.

Citations

Feb 13, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xingang WangJie Chao
Jun 2, 2016·BMC Cardiovascular Disorders·Xing-Han TianMeng Chen
Oct 27, 2016·Particle and Fibre Toxicology·Haijun LiuJie Chao
Nov 28, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Wei ZhangJie Chao
Oct 27, 2018·Molecular Medicine Reports·Pengcheng XingDonglian Wang
May 21, 2017·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Wen ZhuCun-Zhi Liu
Nov 27, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Haijun LiuJie Chao
Dec 28, 2019·Journal of Functional Biomaterials·Eleonora Scarcello, Dominique Lison
Feb 9, 2021·Cardiology Research and Practice·Chen-Xi WangGuo-Liang Yan
Apr 4, 2021·International Journal of Molecular Sciences·Sai Sahana Sundararaman, Emiel P C van der Vorst

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