The role of mex-gene products in antibiotic extrusion in Pseudomonas aeruginosa

Biochemical and Biophysical Research Communications
H YoneyamaT Nakae

Abstract

The antibiotic extrusion machinery in Pseudomonas aeruginosa is assembled from the mex-operon encoded proteins, OprM and MexA-MexB, connecting the outer and inner membranes. To envisage the role of these proteins in antibiotic extrusion and resistance, we employed the gene replacement technique to construct mutants deficient in mexA, mexB, or oprM, and all possible combinations of these genes. Using the Southern and the Western blotting methods, we confirmed that only the target genes were disrupted. All the mutants deficient in OprM exhibited a 4 to 16 times higher susceptibility against quinolone antibiotics, chloramphenicol, and gentamicin than the parent strain. The mutants deficient in MexA or MexB or both MexA and MexB were only 2 to 4 times more susceptible to these antibiotics than the parent strain. All the mutants lacking MexA, MexB, or OprM showed stereospecific hypersusceptibility to beta-lactam antibiotics than the parent strain. However, the extent of susceptibility to each beta-lactam was comparable among the mutants. Strains lacking OprM accumulated the highest level of ciprofloxacin among all these isogenic strains. The strains lacking either MexA or MexB accumulated lower levels of ciprofloxacin than the mutan...Continue Reading

References

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Citations

Oct 22, 2008·Journal of Endourology·Geoffrey R WignallPeter A Cadieux
Aug 5, 2008·Antimicrobial Agents and Chemotherapy·Makoto SugimuraTaiji Nakae
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·N MasudaT Nishino
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·N MasudaT Nishino
Jun 19, 2003·Journal of Bacteriology·Sylvia FrankeDietrich H Nies
May 24, 2006·Bioscience, Biotechnology, and Biochemistry·Hiroshi Yoneyama, Ryoichi Katsumata
Dec 17, 2002·The Journal of General and Applied Microbiology·Yuji MoritaTomofusa Tsuchiya
Dec 24, 2010·Journal of Visualized Experiments : JoVE·Forum H Bhatt, Constance J Jeffery
Aug 28, 2009·FEMS Microbiology Letters·Eisaku YoshiharaSayaka Kaitou
Aug 25, 2004·Biochemical and Biophysical Research Communications·Vladislav V MokhonovTaiji Nakae
May 6, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kazuki SatoHiroshi Yoneyama
May 1, 2004·The Journal of Biological Chemistry·Hiroyuki AkamaTaiji Nakae
Jul 12, 2007·The Journal of Trauma·Alexis D ArmourEdward E Tredget
Oct 2, 2002·European Journal of Biochemistry·Eisaku YoshiharaKohjiro Saito
Mar 14, 2007·The Journal of Biological Chemistry·Shin-Ya TanakaHajime Tokuda
Mar 14, 2007·The Journal of Biological Chemistry·Shin-Ichiro Narita, Hajime Tokuda
Sep 23, 1997·Biochemical and Biophysical Research Communications·T KitaharaT Nakae
Apr 16, 1998·Biochemical and Biophysical Research Communications·H YoneyamaT Nakae

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