The role of microRNA-1246 in the regulation of B cell activation and the pathogenesis of systemic lupus erythematosus

Clinical Epigenetics
Shuangyan LuoQianjin Lu

Abstract

The pathogenesis of systemic lupus erythematosus (SLE) has not yet been completely elucidated. One of the hallmarks of SLE is the production of autoantibodies by uncontrolled over-activated B cells. Early B cell factor 1 (EBF1) contributes to the development, activation, and proliferation of B cells through activation of the AKT signaling pathway. Accumulating evidence has demonstrated that several microRNAs (miRNAs) contribute to the pathogenesis of autoimmune diseases through the regulation of B cells in SLE. We aim to investigate the expression patterns of miR-1246 in B cells and its contribution to pathogenesis of SLE. Our results showed that the expression of miR-1246 was significantly decreased in B cells from SLE patients. We verified that miR-1246 specifically targeted the EBF1 messenger RNA (mRNA) by interacting with its 3'-untranslated region (3'-UTR) and regulated the expression of EBF1. Transfection of miR-1246 inhibitors into healthy B cells upregulated the expression of EBF1, enhanced B cell function, and increased the production of B cell surface co-stimulatory molecules CD40, CD80, and CD86. We also observed that abnormal activation of the AKT signaling pathway was associated with decreased P53 expression, leadi...Continue Reading

References

Oct 1, 1996·Immunological Reviews·S W Van GoolJ L Ceuppens
Jun 19, 2001·Current Opinion in Immunology·G A Bishop, B S Hostager
Dec 1, 2001·The Journal of Biological Chemistry·Susmit SuvasJaved N Agrewala
Dec 14, 2004·Immunologic Research·Rahul K Patel, Chandra Mohan
Mar 18, 2005·Annual Review of Immunology·Rebecca J GreenwaldArlene H Sharpe
Apr 28, 2007·Science·Antony RodriguezAllan Bradley
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicholas W Kin, Virginia M Sanders
Aug 30, 2008·Seminars in Immunology·Kara LukinJames Hagman
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Nov 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Friederike C RauNicole Baumgarth
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Oct 12, 2010·Nature Immunology·Anna-Barbara StittrichMir-Farzin Mashreghi
Jun 2, 2011·Advances in Experimental Medicine and Biology·Biola M Javierre, Bruce Richardson
Aug 9, 2011·Nature Reviews. Cancer·Marieke van KouwenhoveReuven Agami
Aug 14, 2012·Frontiers in Immunology·Jose J Limon, David A Fruman
Oct 17, 2012·Nature Reviews. Rheumatology·Nan ShenPaul-Peter Tak
Jan 24, 2013·Journal of Autoimmunity·Ming ZhaoQianjin Lu
Feb 27, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pandelakis A KoniMichiko Shimoda
Mar 29, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Marianne Frieri
Apr 5, 2013·Immunological Reviews·Virginia G de YébenesAlmudena R Ramiro
Jun 19, 2013·International Journal of Rheumatic Diseases·Xiaochun Ma, Qiji Liu
Sep 4, 2013·Clinical & Developmental Immunology·Nien Yee Kow, Anselm Mak
Oct 17, 2013·Autoimmunity·Aijing Liu, Antonio La Cava
Nov 1, 2013·Kidney International·Jackie A FretzMark C Horowitz
Apr 8, 2014·PloS One·Hiroko Ogata-KawataNaoto Tsuchiya
Apr 26, 2014·Seminars in Immunopathology·Ole Petter Rekvig, Johan Van der Vlag
Jul 8, 2014·Immune Network·Sheng YanVera Sau-Fong Chan
Jul 11, 2014·Current Opinion in Rheumatology·Yun Deng, Betty P Tsao

❮ Previous
Next ❯

Citations

Aug 1, 2015·International Journal of Molecular Sciences·Isabelle Duroux-RichardFlorence Apparailly
May 20, 2015·International Journal of Molecular Sciences·Haijing WuQianjin Lu
Jan 14, 2016·Autoimmunity·Saeed AslaniMohammad Hossein Nicknam
Apr 26, 2016·Journal of Immunology Research·Juan ShiXiaoming Liu
Jul 28, 2016·Experimental Cell Research·Lian-Ju LiDong-Qing Ye
Oct 26, 2016·Journal of Autoimmunity·Hai LongQianjin Lu
Jan 10, 2018·Frontiers in Immunology·Yonglu GongShengjun Wang
Jun 7, 2018·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Yusuke IshibeNaoto Tamura
Jan 30, 2018·Cellular & Molecular Immunology·Haijing WuQianjin Lu
Jul 4, 2019·PloS One·Juliana Carvalhães Lago, Maria Beatriz Puzzi
Mar 20, 2020·International Journal of Molecular Sciences·Chary Lopez-PedreraCarlos Pérez-Sánchez
Mar 24, 2016·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Marketa Husakova
Oct 16, 2019·Frontiers in Immunology·Haijing WuQianjin Lu
Jun 26, 2020·Biomolecules·Mohammad TaheriSoudeh Ghafouri-Fard
Feb 23, 2020·Mediators of Inflammation·Silvia Méndez-FloresJoaquín Zúñiga
Oct 17, 2018·International Journal of Molecular Sciences·Mirco Di MarcoRosa Visone
Oct 23, 2020·Cells·Yuliya Andreevna VeryaskinaIgor Fyodorovich Zhimulev
May 18, 2020·Trends in Microbiology·Riti SharanDeepak Kaushal
Sep 16, 2021·Immunological Reviews·Timothy C BorbetSergei B Koralov
Aug 17, 2021·Nutrition Research Reviews·Tatiana MontoyaCatalina Alarcón-de-la-Lastra

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
flow cytometry
immunoprecipitation
PCR
protein assay
transfections

Software Mentioned

Gene Real - Time Analysis
Rotor
TargetScan
SPSS
miRBase
Quantity One
CellQuest

Related Concepts

Related Feeds

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Alexander EspinosaMarie Wahren-Herlenius
Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Shaoxian HuPeigen He
© 2021 Meta ULC. All rights reserved