The role of miRNAs 34a, 146a, 320a and 542 in the synergistic anticancer effects of methyl 2-(5-fluoro-2-hydroxyphenyl)-1H- benzo[d]imidazole-5-carboxylate (MBIC) with doxorubicin in breast cancer cells

PeerJ
Mohadeseh HasanpourghadiMohd Rais Mustafa

Abstract

Combination Index (CI) analysis suggested that MBIC and doxorubicin synergistically inhibited up to 97% of cell proliferation in ER+/PR+MCF-7 and triple negative MDA-MB-231 breast cancer cell lines. Moreover, treatment of the breast cancer cells with the combined drugs resulted in lower IC50 values in contrast to the individual drug treatment. Small noncoding microRNAs (miRNA) may function as non-mutational gene regulators at post-transcriptional level of protein synthesis. In the present study, the effect of the combined treatment of MBIC and doxorubicin on the expression level of several miRNAs including miR-34a, miR-146a, miR-320a and miR-542 were evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines. These miRNAs have the potential to alter the protein level of survivin, the anti-apoptotic protein and reduce the metastatic activity in human breast cancer cell lines by interfering with the nuclear accumulation of NF-κB. Our results demonstrated the several fold changes in expression of miRNAs, which is drug and cell line dependent. This finding demonstrated a functional synergistic network between miR-34a, miR-320a and miR-542 that are negatively involved in post-transcriptional regulation of survivin in MCF-7 cells. Wh...Continue Reading

References

Jun 2, 2007·Molecular Cell·Nina Raver-ShapiraMoshe Oren
Nov 22, 2007·Trends in Molecular Medicine·Taro Kawai, Shizuo Akira
Jan 12, 2008·Molecular Cancer Therapeutics·Paul E BlowerWolfgang Sadee
Mar 19, 2008·Molecular Cancer Therapeutics·Michael SunRazelle Kurzrock
Jul 28, 2009·International Journal of Cancer. Journal International Du Cancer·Tongsen ZhengLianxin Liu
Apr 13, 2010·Toxicology and Applied Pharmacology·Supratim ChoudhuriCurtis D Klaassen
Apr 24, 2010·Developmental Cell·Olivier Gavet, Jonathon Pines
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Aug 24, 2010·FEBS Letters·Sena YoonKwanghee Baek
Sep 3, 2010·Cancer Research·Andreas G BaderMatthew Winkler
Sep 3, 2010·Blood·Christofer DiakosJoseph L Wiemels
Mar 15, 2011·Breast Cancer Research and Treatment·L KastlA C Schofield
Jul 29, 2011·Biopharmaceutics & Drug Disposition·Alice C RodriguesAi-Ming Yu
Jan 12, 2012·Current Oncology Reports·David N Church, Denis C Talbot
Feb 14, 2012·Pharmaceutical Statistics·J L Sebaugh
Jul 20, 2012·Biochemistry Research International·Eleftheria TsakalozouYounsoo Bae
Dec 25, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Weiguo CaoYunlin Wu
Apr 10, 2013·Expert Opinion on Drug Metabolism & Toxicology·Igor P Pogribny, Frederick A Beland
Feb 18, 2014·Genes & Development·Nobuhiro OkadaLin He
Jul 18, 2014·Cell Death & Disease·X J LiJ H Tang
Sep 16, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sanjun ShiXun Sun
Jul 16, 2015·PloS One·Francisco Navarro, Judy Lieberman
Sep 18, 2015·Nature Protocols·Michelle RichnerAndrew S Yoo

❮ Previous
Next ❯

Citations

Nov 23, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Amira SalehWaleed Barakat

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
nuclear translocation

Software Mentioned

Graph Pad
eqnarray
imageJ
GraphPad Prism

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.