The role of myeloid cell-derived PDGF-B in neotissue formation in a tissue-engineered vascular graft

Regenerative Medicine
Ekene OnwukaChristopher K Breuer

Abstract

Inflammatory myeloid lineage cells mediate neotissue formation in tissue-engineered vascular grafts, but the molecular mechanism is not completely understood. We examined the role of vasculogenic PDGF-B in tissue-engineered vascular graft neotissue development. Myeloid cell-specific PDGF-B knockout mice (PDGF-KO) were generated using bone marrow transplantation, and scaffolds were implanted as inferior vena cava interposition grafts in either PDGF-KO or wild-type mice. After 2 weeks, grafts from PDGF-KO mice had more remaining scaffold polymer and less intimal neotissue development. Increased macrophage apoptosis, decreased smooth muscle cell proliferation and decreased collagen content was also observed. Myeloid cell-derived PDGF contributes to vascular neotissue formation by regulating macrophage apoptosis, smooth muscle cell proliferation and extracellular matrix deposition.

References

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Citations

Jan 11, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Randall J SmithStelios T Andreadis
Mar 20, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Lin ChangY Eugene Chen
Apr 3, 2020·Nature Communications·Randall J SmithStelios T Andreadis
Apr 15, 2021·Scientific Reports·Kevin M BlumChristopher K Breuer
Jan 1, 2020·Journal of Vascular Surgery. Vascular Science·Hideki MiyachiToshiharu Shinoka

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Methods Mentioned

BETA
genotyping
PCR
dissection
gene knockout
light microscopy

Software Mentioned

GraphPad
GraphPad Prism
ImageJ

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