The role of Na+/K+-ATPase during chick skeletal myogenesis

PloS One
Taissa Neustadt OliveiraCláudia Mermelstein

Abstract

The formation of a vertebrate skeletal muscle fiber involves a series of sequential and interdependent events that occurs during embryogenesis. One of these events is myoblast fusion which has been widely studied, yet not completely understood. It was previously shown that during myoblast fusion there is an increase in the expression of Na+/K+-ATPase. This fact prompted us to search for a role of the enzyme during chick in vitro skeletal myogenesis. Chick myogenic cells were treated with the Na+/K+-ATPase inhibitor ouabain in four different concentrations (0.01-10 μM) and analyzed. Our results show that 0.01, 0.1 and 1 μM ouabain did not induce changes in cell viability, whereas 10 μM induced a 45% decrease. We also observed a reduction in the number and thickness of multinucleated myotubes and a decrease in the number of myoblasts after 10 μM ouabain treatment. We tested the involvement of MEK-ERK and p38 signaling pathways in the ouabain-induced effects during myogenesis, since both pathways have been associated with Na+/K+-ATPase. The MEK-ERK inhibitor U0126 alone did not alter cell viability and did not change ouabain effect. The p38 inhibitor SB202190 alone or together with 10 μM ouabain did not alter cell viability. Our r...Continue Reading

References

Aug 25, 1977·Nature·J Prives, M Shinitzky
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M HamlynJ H Ludens
Dec 1, 1987·Developmental Biology·Z Kaprielian, D M Fambrough
Jan 1, 1994·In Vitro Cellular & Developmental Biology. Animal·P G Pauw, G J Hermann
Nov 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S NamciuE Stavnezer
May 30, 1998·The Journal of Biological Chemistry·E GredingerE Bengal
Mar 16, 2000·In Vitro Cellular & Developmental Biology. Animal·P G PauwD C Williams
Aug 10, 2000·Biochemical Pharmacology·L E QuintasF Noël
Apr 25, 2001·Journal of Cellular Physiology·O Sharabani-YosefS R Sampson
Nov 7, 2002·Biochemical and Biophysical Research Communications·Jinhwa LeeSung Soo Kim
Nov 30, 2002·The Journal of Biological Chemistry·Amy E MoseleyJerry B Lingrel
May 10, 2003·Developmental Cell·Martin SchwanderUlrich Müller
May 24, 2003·Annals of the New York Academy of Sciences·John M HamlynBruce P Hamilton
Feb 14, 2004·The Journal of Biological Chemistry·Haojie WangZijian Xie
Nov 26, 2005·Domestic Animal Endocrinology·Pascal VaudinSophie Tesseraud
Apr 29, 2006·Molecular and Cellular Endocrinology·Aviad KerenEyal Bengal
May 30, 2006·Biochemical and Biophysical Research Communications·Ju Li, Sally E Johnson
Feb 14, 2007·The Journal of Biological Chemistry·Man LiangZi-Jian Xie
Mar 16, 2007·Differentiation; Research in Biological Diversity·Cláudia S MermelsteinJosé Garcia Abreu
Sep 17, 2008·The Journal of Cell Biology·Ting CaiZi-Jian Xie
Mar 28, 2009·Pharmacological Reviews·Alexei Y BagrovOlga V Fedorova
Apr 14, 2009·The Journal of Biological Chemistry·Yiliang ChenZijian Xie
May 11, 2010·Journal of Molecular and Cellular Cardiology·Luis E M QuintasZi-Jian Xie
Sep 3, 2010·Annual Review of Physiology·Warren Thomas, Brian J Harvey
Jan 13, 2012·American Journal of Physiology. Renal Physiology·Francisco J Arnaud-BatistaNilberto R F Nascimento
Jan 21, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Clay E PandorfFadia Haddad

❮ Previous
Next ❯

Citations

Apr 7, 2017·American Journal of Physiology. Cell Physiology·Aline M S YamashitaLeonardo Nogueira
Sep 19, 2018·International Journal of Molecular Sciences·Luciana S AmaralLuis Eduardo M Quintas
Jun 8, 2021·Frontiers in Physiology·Manoel L CostaClaudia Mermelstein

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

© 2021 Meta ULC. All rights reserved