The role of nitric oxide in reducing deformability of Lewis lung tumor cell stimulated by inflammatory cytokines

Nitric Oxide : Biology and Chemistry
Kenichi KokuboHirosuke Kobayashi

Abstract

Highly metastatic cells, especially in the lungs, are known to be resistant to nitric oxide (NO)-mediated cytotoxicity, compared with poorly or non-metastatic cells. However, the precise mechanisms connecting NO and metastasis remain to be determined. To clarify the role of NO in the characteristic changes in NO-resistant cells in response to inflammatory cytokines, we used Lewis lung tumor (LLT) cells, which are known to be highly metastatic NO-resistant cells, and determined the changes in cell deformability and the gene expression profile after the cells were stimulated using cytokine mixture or an NO donor. Both exogenous NO and endogenous NO via inducible NO synthase produced by cytokines decreased cell deformability by enhancing actin polymerization. The expression of several genes associated with actin polymerization was changed so as to increase actin filaments in the cells by enhancing actin polymerization and by suppressing actin depolymerization, actin filament severing, and barbed-end actin filament capping. In conclusion, inflammatory cytokine stimulation reduces deformability of LLT cells and enhances actin polymerization which is mainly controlled by the same genes induced by NO.

References

Jul 1, 1988·Journal of Leukocyte Biology·I J AmberZ Vavrin
Nov 1, 1983·British Journal of Cancer·D Glaves
Sep 16, 1980·Biochemical and Biophysical Research Communications·S MacLean-Fletcher, T D Pollard
Nov 3, 1995·International Journal of Cancer. Journal International Du Cancer·M RochaV Umansky
Feb 23, 1996·Biochemical Pharmacology·G J Southan, C Szabó
Jun 1, 1996·Journal of Leukocyte Biology·K XieI J Fidler
May 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S AmbsC C Harris
Apr 17, 1998·Cancer Metastasis Reviews·K Xie, I J Fidler
Feb 27, 1999·Current Opinion in Cell Biology·P Aspenström
Nov 24, 1999·The Journal of Biological Chemistry·H Q SunH L Yin
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Sep 13, 2000·Life Sciences·M El MabroukE L Schiffrin
Jul 13, 2001·Advances in Cancer Research·V Umansky, V Schirrmacher
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Judith H HarmeyZhongyun Dong
Oct 17, 2002·Nitric Oxide : Biology and Chemistry·Hirosuke KobayashiTomoyuki Tomita
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Feb 5, 2003·The Journal of Cell Biology·Raymond S MaulDavid D Chang
Apr 10, 2003·Free Radical Biology & Medicine·Keping Xie, Suyun Huang
Mar 9, 2004·Molecular Biology of the Cell·Enni BertlingPekka Lappalainen
Apr 10, 2004·Cancer Science·Satoshi IgawaHirosuke Kobayashi
Sep 12, 2006·Nature Biotechnology·Roger D CanalesFederico M Goodsaid
Feb 20, 2008·The Journal of Biological Chemistry·Stephen R ThomTatyana N Milovanova

❮ Previous
Next ❯

Citations

Apr 13, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Zhuolong ZhouAnxun Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.