The role of nitric oxide in the epigenetic regulation of THP-1 induced by lipopolysaccharide

Life Sciences
Ester Correia Sarmento RiosFrancisco Garcia Soriano

Abstract

Changes in the gene expression are one of the molecular events involved in the Systemic of Inflammatory Response Syndrome during sepsis. The preconditioning with low doses of lipopolysaccharide (LPS) reduces the expression of pro-inflammatory genes leading to less tissue damage and better outcome. This hyporesponsive state called tolerance is associated to alterations in chromatin structure and nitric oxide (NO) production. In the current study, we demonstrated that tolerance induced by LPS was found to be NO-dependent and related to epigenetic changes. THP-1 cells were cultivated in RPMI medium (Control), submitted to tolerance (500ng/mL of LPS 24h before challenge with 1000ng/mL of LPS during 24h Tolerant group) and challenge (1000ng/mL of LPS during 24h Directly challenged group). The analyses performed were: cytokines production, histone acetyl transferases/histone deacetylases (HAT/HDAC) activity, nitrosylation of HDAC-2 and -3, expression of acetylated histones H3 and H4. HDAC and Nitric Oxide Synthases (NOS) activities were inhibited with 30mM trichostatin (TSA) and 100μM LNAME, respectively. Administration of low doses of LPS repressed the production of IL-6 and IL-10, however this effect was abolished with the inhibiti...Continue Reading

References

Apr 16, 1998·Genes & Development·K Struhl
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SunG Meissner
Jan 10, 2002·Critical Care Medicine·Michael A West, Wyrta Heagy
Jul 26, 2002·Journal of the American Society of Nephrology : JASN·Zhiyuan YuBruce C Kone
Jan 23, 2003·British Journal of Anaesthesia·J MacdonaldN R Webster
Feb 4, 2003·Nature Reviews. Immunology·Bruce Beutler, Ernst Th Rietschel
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Douglas T HessJonathan S Stamler
Nov 18, 2005·Gene·Michele A GlozakEdward Seto
Jan 24, 2006·Molecular Cell·Antonella RiccioDavid D Ginty
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yasuko IwakiriWilliam C Sessa
Jun 1, 2010·Endocrine, Metabolic & Immune Disorders Drug Targets·Clara Batista LorigadosCsaba Szabo
Aug 24, 2010·Molecular Immunology·Edielle Sant'Anna MeloFrancisco Garcia Soriano
Oct 26, 2010·Nature Cell Biology·Michael D KornbergSolomon H Snyder
Mar 1, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Puneet Anand, Jonathan S Stamler
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alexi NottAntonella Riccio

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Citations

Apr 25, 2019·Journal of Medical Virology·Xiaolin DingBo Qin
Jan 19, 2019·International Journal of Molecular Sciences·Andreas von Knethen, Bernhard Brüne
Jul 30, 2021·Frontiers in Medicine·Renata Brito Falcão-HolandaReinaldo Salomão

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