The role of nuclear factor erythroid-2-related factor 2 expression in radiocontrast-induced nephropathy

Scientific Reports
Jieun KimGang Jee Ko

Abstract

Radiocontrast-induced nephropathy (CIN) is the third most common cause of acute renal failure. The pathophysiology of CIN is related to tubular injury caused by oxidative stress, and nuclear factor erythroid-2-related factor 2 (Nrf2) is critical in coordinating intracellular antioxidative processes. We thus investigated the role of Nrf2 in CIN. CIN was established in mice and in NRK-52E cells via iohexol administration according to the protocols of previous studies. To determine the role of Nrf2 in CIN, Nrf2 expression was reduced in vivo using Nrf2 knockout (KO) mice (B6.129 × 1-Nfe2 l2tm1Ywk/J) and in vitro with siRNA treatment targeting Nrf2. Increased Nrf2 expression was observed after iohexol treatment both in vivo and in vitro. Serum creatinine at 24 h after iohexol injection was significantly higher in KO mice than in wild-type (WT) mice. Histologic examination showed that iohexol-induced tubular vacuolization and structural disruption were aggravated in Nrf2 KO mice. Significant increases in apoptosis and F4/80(+) inflammatory cell infiltration were demonstrated in KO mice compared to WT mice. In addition, the increase in reactive oxygen species after iohexol treatment was augmented by Nrf2 inhibition both in vivo and i...Continue Reading

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Citations

Sep 17, 2020·International Journal of Molecular Sciences·Yu Ah HongGang-Jee Ko
Feb 12, 2021·Phytotherapy Research : PTR·Marjan TalebiSaeed Samarghandian

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Methods Mentioned

BETA
transfection
MDA
confocal microscopy
genotyping
PCR
ELISA
electrophoresis

Software Mentioned

ImageJ
Image
Pro Plus
SPSS
Zen2009

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