The role of O-linked and N-linked oligosaccharides on the structure-function of glycoprotein hormones: development of agonists and antagonists

Biochimica Et Biophysica Acta
F A Fares

Abstract

Thyrotropin (TSH) and the gonadotropins; follitropin (FSH), lutropin (LH) and human chorionic gonadotropin (hCG) are a family of heterodimeric glycoprotein hormones. These hormones composed of two noncovalently linked subunits; a common alpha and a hormone specific beta subunits. Assembly of the subunits is vital to the function of these hormones. However, genetic fusion of the alpha and beta subunits of hFSH, hCG and hTSH resulted in active polypeptides. The glycoprotein hormone subunits contain one (TSH and LH) or two (alpha, FSHbeta and hCGbeta) asparagine-linked (N-linked) oligosaccharides. CGbeta subunit is distinguished among the beta subunits because of the presence of a carboxyl-terminal peptide (CTP) bearing four O-linked oligosaccharide chains. To examine the role of the oligosaccharide chains on the structure-function of glycoprotein hormones, chemical, enzymatic and site-directed mutagenesis were used. The results indicated that O-linked oligosaccharides play a minor role in receptor binding and signal transduction of the glycoprotein hormones. In contrast, the O-linked oligosaccharides are critical for in vivo half-life and bioactivity. Ligation of the CTP bearing four O-linked oligosaccharide sites to different pr...Continue Reading

References

Jan 1, 1979·The Journal of Endocrinology·J R SowersJ J DiStefano
Oct 15, 1992·European Journal of Biochemistry·A Kobata
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A FaresI Boime
Feb 1, 1990·Endocrine Reviews·S D GharibW W Chin
Jan 15, 1990·Biochemical and Biophysical Research Communications·M MisrahiE Milgrom
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V B ReddyE G Bernstine
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K KozarskyM Krieger
Dec 31, 1987·Biochemical and Biophysical Research Communications·S WatanabeK Matsubara
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M M MatzukI Boime
Sep 2, 1985·FEBS Letters·Y HayashizakiK Matsubara
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Oct 15, 1982·Experientia·E G BergerJ F Vliegenthart
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T SugaharaI Boime
Jun 9, 1994·Nature·A J LapthornN W Isaacs
Oct 1, 1996·Nature Biotechnology·M W SzkudlinskiB D Weintraub
Nov 18, 2000·The Journal of Biological Chemistry·F A FaresZ Kraiem
Feb 27, 2001·Molecular Endocrinology·K M FoxP Van Roey
Jul 28, 2001·Human Reproduction·P M BoulouxUNKNOWN FSH-CTP study group
Mar 28, 2002·Physiological Reviews·Mariusz W SzkudlinskiBruce D Weintraub
Jan 15, 2003·Human Reproduction·J KleinJ W Lustbader
Jul 5, 2003·The Journal of Clinical Endocrinology and Metabolism·Signe PerlmanHans T Schambye

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Citations

Oct 24, 2008·Human Reproduction Update·S F de Medeiros, R J Norman
May 9, 2009·BMC Cell Biology·Jeroen R P M Strating, Gerard J M Martens
Mar 24, 2007·The Journal of Reproduction and Development·Zhengchao WangKazuyoshi Taya
Oct 1, 2013·Thyroid : Official Journal of the American Thyroid Association·Joshua M EstradaOffie P Soldin
Feb 25, 2009·Immunopharmacology and Immunotoxicology·Annalisa RizzoRaffaele Sciorsci
Sep 13, 2011·General and Comparative Endocrinology·Rafael Vázquez-MartínezMaría M Malagón
Nov 1, 2015·General and Comparative Endocrinology·Mitsuo NyujiKoichi Okuzawa
Apr 17, 2007·Animal Reproduction Science·G Perera-MarínE González-Padilla
Oct 1, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·François ChauvignéJoan Cerdà
Jan 27, 2009·Journal of Chromatography. a·C M CarvalhoM T C P Ribela
Jan 30, 2008·Protein Science : a Publication of the Protein Society·Seiji SaitoShunichi Shimasaki
May 30, 2012·Proteomics. Clinical Applications·Qing Kay LiHui Zhang
Dec 8, 2010·Proteomics. Clinical Applications·Yuan Tian, Hui Zhang
Dec 3, 2014·General and Comparative Endocrinology·François ChauvignéJoan Cerdà
Oct 7, 2015·Frontiers in Endocrinology·Selvaraj G NatarajaStephen S Palmer
Sep 5, 2013·Molecular and Cellular Endocrinology·Xuliang JiangXiaolin He
Jul 18, 2016·Carbohydrate Research·Mao-Mao WangJosef Voglmeir
Nov 23, 2016·Journal of Molecular Endocrinology·Ricardo Núñez MiguelBernard Rees Smith
Jun 13, 2009·Human Reproduction·Kevin K W LamWilliam S B Yeung
Apr 19, 2017·The Journal of Clinical Endocrinology and Metabolism·Marta GarcíaJosé C Moreno
Jul 10, 2018·Endocrine Reviews·Ross C AndersonRobert P Millar
Jul 9, 2016·Molecular Endocrinology·Gunnar KleinauAnnette Grüters-Kieslich
May 7, 2013·Endocrine Reviews·Gunnar KleinauHeike Biebermann
Mar 29, 2020·International Journal of Molecular Sciences·Livio CasariniManuela Simoni
Jul 26, 2018·Cancer Gene Therapy·Zinaida A DubeykovskayaTimothy C Wang
Nov 30, 2018·Protein Engineering, Design & Selection : PEDS·Wu-Mei YuanLi-Shu Zheng
Jan 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lars AndresenSøren Skov
May 6, 2020·Journal of Assisted Reproduction and Genetics·Lixia ZhuLei Jin

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