The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function

Toxicology in Vitro : an International Journal Published in Association with BIBRA
V StoneK Donaldson

Abstract

Respired ultrafine particles induce a greater inflammation in rat lungs than fine particles; we have hypothesized that this is due to their comparatively huge number and surface area for the production of free radicals. We tested this hypothesis by studying the effects of fine and ultrafine (uf) carbon black (CB) particles in comparison with quartz on A549 human type II alveolar epithelial cells, particularly with respect to the oxidative properties of these particles. Treatment with fine CB (diameter 260nm), and quartz (up to 0.78mug/mm(2)) for 24 hours significantly (P<0.05) decreased the A549 cells metabolic competence, as measured by the ability to reduce MTT to a formazan product. The inhibitory effects of uf CB only became significantly different (P<0.05) relative to the control at 48 hours, by which time the effects of fine CB and quartz were no longer significant. The inhibition of MTT reduction by uf CB was prevented by the hydroxyl radical scavenger mannitol (2mm). In addition, measurement of reactive oxygen species production using supercoiled plasmid DNA showed that uf CB exhibited significantly more free radical activity than fine CB (P<0.05). In the absence of serum, uf CB depleted reduced glutathione at 6 hours (...Continue Reading

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Citations

Jan 1, 2003·Environmental Health and Preventive Medicine·Vicki StoneKenneth Donaldson
Sep 12, 2007·Cell Biology and Toxicology·Peter G BarlowVicki Stone
Mar 27, 2010·Cell Biology and Toxicology·Flavia Mazzoli-RochaWalter Araújo Zin
Feb 14, 2004·Toxicology and Applied Pharmacology·Roel P F SchinsVicki Stone
May 22, 2003·Free Radical Biology & Medicine·Ken DonaldsonWilliam MacNee
Dec 16, 2011·Journal of Occupational and Environmental Hygiene·Peter C RaynorJames B D'Arcy
Aug 20, 2002·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·Ken DonaldsonVicki Stone
Aug 6, 2004·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·Wolfgang G KreylingWinfried Möller
Aug 10, 2000·American Journal of Respiratory and Critical Care Medicine·V Stone
Dec 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Huijung KimStephen I Rennard
Dec 23, 2004·Respiratory Research·D M BrownV Stone
Jul 9, 2013·Nanoscale Research Letters·Mona ConnollyJosé M Navas
Oct 26, 2005·Particle and Fibre Toxicology·Ken DonaldsonVicki Stone
Mar 5, 2011·Particle and Fibre Toxicology·Igor PujaltéBéatrice L'Azou
Sep 13, 2013·BioMed Research International·Amruta MankeYon Rojanasakul
Nov 13, 2013·Annual Review of Physiology·Amy K MadlKent E Pinkerton
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Aug 1, 2014·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Jiayuan Zhao, Michael Riediker
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Jul 20, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L FoucaudJ Falla
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Jan 26, 2008·IEEE Transactions on Nanobioscience·Vicki StoneMartin J D Clift
Jan 3, 2006·Toxicology and Applied Pharmacology·Shoji YamamotoHidekazu Fujimaki
Jun 19, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Matthew S P BoylesVicki Stone
Jul 15, 2015·Environmental Science. Processes & Impacts·Hua ZouMeibian Zhang
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May 13, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Helinor JohnstonVicki Stone
Jan 3, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Quaiser SaquibJaved Musarrat
Feb 7, 2015·Environmental Science. Processes & Impacts·Mingluan XingMeibian Zhang
Apr 12, 2001·Toxicology and Applied Pharmacology·L C RenwickA Clouter

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