The Role of Peroxisome Proliferator–Activated Receptor Gamma (PPARγ) in Mono(2-ethylhexyl) Phthalate (MEHP)-Mediated Cytotrophoblast Differentiation

Environmental Health Perspectives
Hussein ShoaitoSéverine A Degrelle

Abstract

Phthalates are environmental contaminants commonly used as plasticizers in polyvinyl chloride (PVC) products. Recently, exposure to phthalates has been associated with preterm birth, low birth weight, and pregnancy loss. There is limited information about the possible mechanisms linking maternal phthalate exposure and placental development, but one such mechanism may be mediated by peroxisome proliferator–activated receptor γ (PPARγ). PPARγ belongs to the nuclear receptor superfamily that regulates, in a ligand-dependent manner, the transcription of target genes. Studies of PPARγ-deficient mice have demonstrated its essential role in lipid metabolism and placental development. In the human placenta, PPARγ is expressed in the villous cytotrophoblast (VCT) and is activated during its differentiation into syncytiotrophoblast. The goal of this study was to investigate the action of mono(2-ethylhexyl) phthalate (MEHP) on PPARγ activity during in vitro differentiation of VCTs. We combined immunofluorescence, PPARγ activity/hCG assays, western blotting, and lipidomics analyses to characterize the impacts of physiologically relevant concentrations of MEHP (0.1, 1, and 10 μM) on cultured VCTs isolated from human term placentas. Doses of...Continue Reading

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Citations

Feb 28, 2020·International Journal of Molecular Sciences·Zi-Run TangKun Yu
Sep 24, 2020·International Journal of Environmental Research and Public Health·Henrieta HlisníkováAlexander Sirotkin
Aug 31, 2020·Epigenetics Insights·Laurie K SvobodaDana C Dolinoy
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May 24, 2021·Environmental Science and Pollution Research International·Hamidreza Mohammadi, Sorour Ashari
May 21, 2021·Reproductive Toxicology·Genoa R WarnerJones B Graceli
Aug 17, 2021·Journal of Hazardous Materials·Cong-Cong SunLing-Li Zhao
Aug 30, 2021·Environment International·Minghui HanQingwu Jiang

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Methods Mentioned

BETA
cesarean section
light microscopy
Assay
FCS
transfection
Protein Assay

Software Mentioned

Icy
qBasePlus
ImageJ
GraphPad Prism
Excel
P +
DA
OPLS
SIMCA
Waters [UNK]

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