The role of phosphodiesterase isoforms 2, 5, and 9 in the regulation of NO-dependent and NO-independent cGMP production in the rat cervical spinal cord

Journal of Chemical Neuroanatomy
J De VenteJ S Vles

Abstract

NO-responsive, cGMP-producing structures are abundantly present in the cervical spinal cord. NO-mediated cGMP synthesis has been implicated in nociceptive signaling and it has been demonstrated that cGMP has a role establishing synaptic connections in the spinal cord during development. As cGMP levels are controlled by the activity of soluble guanylyl cyclase (synthesis) and the phosphodiesterase (PDE) activity (breakdown), we studied the influence of PDE activity on NO-stimulated cGMP levels in the rat cervical spinal cord. cGMP-immunoreactivity (cGMP-IR) was localized in sections prepared from slices incubated in vitro. A number of reported PDE isoform-selective PDE inhibitors was studied in combination with diethylamineNONOate (DEANO) as a NO-donor including isobutyl-methylxanthine (IBMX) as a non-selective PDE inhibitor. We studied 8-methoxy-IBMX as a selective PDE1 inhibitor, erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) and BAY 60-7550 as selective PDE2 inhibitors, sildenafil as a selective PDE5 inhibitor, dipyridamole as a mixed type PDE5 and PDE10 inhibitor, rolipram as a PDE4 inhibitor, and SCH 81566 as a selective PDE9 inhibitor. cGMP-IR structures (nerve fibers, axons, and terminals) were characterized using the follow...Continue Reading

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Citations

Aug 19, 2010·Pharmacological Reviews·Sharron H FrancisDavid Sibley
Jun 10, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Diane T StephensonDaniel Morton
Aug 7, 2013·Acta neurologica Belgica·Murat AltaşN Yılmaz
Jan 19, 2010·European Journal of Pharmacology·Claudia I Araiza-SaldañaVinicio Granados-Soto
Oct 31, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Yurdal SerarslanTaşkin Yurtseven
May 6, 2009·Trends in Neurosciences·Achim SchmidtkoGerd Geisslinger
Mar 4, 2009·Pharmacology, Biochemistry, and Behavior·Myung Ha YoonLan Ji Huang
Apr 20, 2006·Journal of Chemical Neuroanatomy·J de VenteJ S H Vles
Dec 7, 2014·World Journal of Urology·M S Rahnama'iG A van Koeveringe
Aug 23, 2011·Neuropharmacology·Joshua S RodeferSamuel J Eckrich
May 12, 2010·Journal of Veterinary Science·Woong Mo KimJin Hua Cui
Feb 14, 2012·The Journal of Pharmacology and Experimental Therapeutics·Robin J KleimanChristopher J Schmidt
Mar 3, 2020·The European Journal of Neuroscience·Sarah E GartsideBas M J Olthof

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