The role of PI 3-kinase in EGF-stimulated jejunal glucose transport

Canadian Journal of Physiology and Pharmacology
Grant A MillarD Grant Gall

Abstract

Epidermal growth factor (EGF) rapidly increases jejunal glucose transport. Signal transduction mechanisms mediating EGF-induced alterations in jejunal glucose transport remain to be determined. New Zealand White rabbit (1 kg) jejunal tissue was stripped and mounted in short-circuited Ussing chambers. The transport of tritiated 3-O-methylglucose was measured in the presence of the PKC agonist 1,2-dioctanoyl-sn-glycerol (1,2-DOG) or the inactive analog 1,3-dioctanoyl-sn-glycerol (1,3-DOG). Additional experiments examined the effect of the PKC inhibitor chelerythrine, the PLC inhibitor U73122, the MAPK inhibitor PD 98059, the G-protein inhibitor GDP-betaS, the PI 3-kinase inhibitor LY294002, or the microtubule inhibitor colchicine on EGF-induced jejunal glucose transport. Net jejunal 3-O-methylglucose absorption was significantly increased following specific activation of PKC. A PKC antagonist inhibited the EGF-induced increase in net 3-O-methylglucose transport, while PI 3-kinase inhibition completely blocked the EGF-induced transport increase. Inhibition of PLC, MAPK, G-proteins, and microtubules had no effect on EGF-stimulated increases in jejunal transport. We conclude that the effect of EGF on jejunal glucose transport is med...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
Nov 15, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Jan 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·U MartiA L Jones
Jun 1, 1972·The Journal of Pediatrics·M KellyJ R Hamilton
Jul 1, 1994·The American Journal of Physiology·N A Bradbury, R J Bridges
Oct 6, 1995·Cell·V M StuditskyG Felsenfeld
May 1, 1995·Cell Biology International·J BoonstraP van Bergen en Henegouwen
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Mar 1, 1994·The American Journal of Physiology·C M FullerD J Benos
Feb 1, 1993·The American Journal of Physiology·J A HardinD G Gall
Sep 1, 1996·The American Journal of Physiology·J A HardinD G Gall
Jul 1, 1997·The Journal of Clinical Investigation·M F SantosG Tigyi
Oct 13, 1998·Current Biology : CB·M M ChouA Toker
Dec 5, 1998·Biochimica Et Biophysica Acta·M P Wymann, L Pirola
Jan 14, 1999·The American Journal of Physiology·T IshizukaK Yasuda
Feb 10, 1999·The American Journal of Physiology·B M ChungD G Gall
May 18, 1999·The American Journal of Physiology·S Vayro, M Silverman
Feb 1, 2000·Journal of Cellular Physiology·V MassheimerA R De Boland
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A N Alexander, H V Carey
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S MarandiJ P Buts
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST

❮ Previous
Next ❯

Citations

Jan 20, 2009·World Journal of Gastroenterology : WJG·Laurie Drozdowski, Alan B R Thomson
Feb 21, 2009·World Journal of Gastroenterology : WJG·Laurie A DrozdowskiAlan B R Thomson
Sep 13, 2014·Journal of Cellular Physiology·María J Rodríguez-YoldiJesús Osada
Nov 11, 2008·The Journal of Nutritional Biochemistry·Laurie A DrozdowskiAlan B R Thomson
Feb 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xue-Lin CuiRonaldo P Ferraris
May 29, 2008·Clinical Orthopaedics and Related Research·David E JoynerR Lor Randall
Nov 15, 2002·Canadian Journal of Physiology and Pharmacology·B M ChungD G Gall
Dec 24, 2011·PloS One·Srikarthika JambunathanVishwajeet Puri
Feb 18, 2016·Oncotarget·Xinjing WangBaiyong Shen
Aug 29, 2006·World Journal of Gastroenterology : WJG·Laurie Drozdowski, Alan B R Thomson

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.