The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis.

Human Molecular Genetics
Lyle ArmstrongMajlinda Lako

Abstract

Understanding the molecular mechanism by which pluripotency is maintained in human embryonic stem cells (hESC) is important for the development of improved methods to derive, culture and differentiate these into cells of potential therapeutic use. Large-scale transcriptional comparison of the hES-NCL1 line derived from a day 8 embryo with H1 line derived from a day 5 embryo (WiCell Inc.) showed that only 0.52% of the transcripts analysed varied significantly between the two cell lines. This is within the variability range that has been reported when hESC derived from days 5-6 embryos have been compared with each other. This implies that transcriptional differences between the cell lines are likely to reflect their genetic profile rather than the embryonic stage from which they were derived. Bioinformatic analysis of expression changes observed when these cells were induced to differentiate as embryoid bodies suggested that quite a few of the downregulated genes were components of signal transduction networks. Subsequent analysis using western blotting, flow cytometry and antibody arrays implicated components of the PI3K/AKT kinase, MAPK/ERK and NFkappabeta pathways and confirmed that these components are decreased upon differen...Continue Reading

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Dec 16, 1998·Science·S TanakaJ Rossant
Aug 19, 2000·Methods in Molecular Biology·T J PetersM J Soares
Feb 12, 2002·International Review of Cytology·Masahito WatanabeHana Hayasaki
Oct 10, 2002·Neuropeptides·Kirill V TarasovKenneth R Boheler
Nov 12, 2002·Nature Biotechnology·Ren-He XuJames A Thomson
Feb 5, 2003·Nature Genetics·Vivian G CheungRichard S Spielman
Feb 11, 2003·Nature Biotechnology·Thomas P Zwaka, James A Thomson
Feb 25, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michal GroppBenjamin E Reubinoff
May 30, 2003·Nature·Kazutoshi TakahashiShinya Yamanaka
Aug 19, 2003·Developmental Biology·Noboru SatoAli H Brivanlou
Oct 24, 2003·BMC Neuroscience·Thomas C SchulzBrian G Condie
Oct 29, 2003·The Journal of Cell Biology·Silvia ParisiGabriella Minchiotti
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jamie M SpergerJames A Thomson
Nov 25, 2003·Biology of Reproduction·M AmitJ Itskovitz-Eldor
Dec 9, 2003·Nature Biotechnology·Jonathan S DraperPeter W Andrews
Dec 23, 2003·Stem Cells·Mark RichardsAriff Bongso
Dec 31, 2003·Biochemical and Biophysical Research Communications·Urara Yoshida-KoideHiroshi Koide
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Melissa K CarpenterMahendra S Rao
Jan 30, 2004·Human Molecular Genetics·Michael J AbeytaMeri T Firpo
Apr 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaoxia QiGuang-Quan Zhao
Apr 28, 2004·Developmental Biology·Irene GinisMahendra S Rao
May 22, 2004·Stem Cells·Xianmin ZengWilliam J Freed
Jul 24, 2004·Immunology·M Fatima MacedoMargarida Correia-Neves
Aug 12, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yingchun WangDaniel A Rappolee
Aug 18, 2004·Genome Research·Naka HattoriKunio Shiota
Aug 26, 2004·The Journal of Biological Chemistry·Nicholas R D PalingMelanie J Welham
Sep 3, 2004·Stem Cells·Laurence DahéronGeorge Q Daley
Sep 4, 2004·Stem Cells and Development·Jonathan S DraperPeter W Andrews
Sep 21, 2004·Oncogene·Ian Chambers, Austin Smith
Jan 27, 2005·Reproductive Biomedicine Online·Nick StrelchenkoYury Verlinsky

❮ Previous
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Citations

Jun 4, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Morag H StewartMickie Bhatia
Nov 16, 2007·Cell and Tissue Research·Stuart Atkinson, Lyle Armstrong
Aug 9, 2007·Journal of Molecular Histology·Martin GrünertVictor Nurcombe
Jan 5, 2013·Journal of Assisted Reproduction and Genetics·Melisa Candela CroccoAdriana Bos-Mikich
Sep 18, 2007·Stem Cell Reviews·Oliver Dreesen, Ali H Brivanlou
Jun 24, 2010·Cell Stem Cell·Kouichi HasegawaMartin F Pera
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yue XuSheng Ding
Jan 5, 2011·Antioxidants & Redox Signaling·Ji Woong Han, Young-Sup Yoon
Sep 16, 2009·Cloning and Stem Cells·Nicholas R F HannanErnst J Wolvetang
Feb 9, 2011·Stem Cells and Development·Suresh RamakrishnaKwang-Hyun Baek
Jul 18, 2008·Human Molecular Genetics·Victoria V Lunyak, Michael G Rosenfeld
Aug 22, 2008·Molecular Human Reproduction·B V JohnsonR A Keough
Jul 6, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jem A Efe, Sheng Ding
Nov 17, 2007·Artificial Organs·Bülend InançY Murat Elçin
Dec 29, 2007·BMC Genomics·Thomas C SchulzXianmin Zeng
Jan 11, 2008·Genome Biology·Randolph S FaustinoAndre Terzic
Jun 29, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Natasha S BartenevaIvan A Vorobjev
May 23, 2012·PloS One·Yubing SunJianping Fu
Jan 26, 2013·PloS One·Masaki KineharaMiho K Furue
Jul 12, 2007·Molecular Medicine·Roberto D FanganielloMaria Rita Passos-Bueno
Feb 19, 2010·Experimental & Molecular Medicine·Ae-Ri JiYoung Min Choi

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