The role of PI3K/Akt signal pathway in the protective effects of propofol on intestinal and lung injury induced by intestinal ischemia/reperfusion1

Acta Cirúrgica Brasileira
Qing-Wen LiXiang-Hu He

Abstract

To investigate the role of PI3k/Akt signal pathway in the protective effects of propofol on intestinal and lung injury induced by intestinal ischemia/reperfusion(I/R). Male Sprague-Dawley rats were subjected to 45 min of ischemia by occluding the superior mesenteric artery and to 2h of reperfusion to establish the model of I/R. Twenty four rats were randomly divided into four groups: Sham, intestinal I/R (II/R), propofol (P), wortmannin (W). In groups P, W, propofol was injected intravenously and continuously at the onset of reperfusion via infusion pump. PI3K inhibitor (wortmannin) was administered intravenously in group W 25 min before ischemia. Intestinal tissues and lung tissues were obtained for determination of histologic injury, wet/dry weight ratio, malondialdehyde (MDA) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. Meanwhile, the expressions of caspase-3 and phosphorylated Akt (p-Akt) in intestines and lungs were detected by western blot. Propofol treatment alleviated intestinal and lung morphological changes which were observed in II/R group,Moreover, wet/dry weight ratio, the MDA level, MPO activity and expression of caspase-3 were significantly decreased whereas the SOD activity and p-Akt ...Continue Reading

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Citations

Jun 25, 2019·Journal of Biomolecular Structure & Dynamics·Wen-Qing JiaXian-Chao Cheng
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Tsai-Chun LaiYuh-Lien Chen
Mar 6, 2021·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Yucel KaramanYalcin Guvenli

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Methods Mentioned

BETA
sedation
SMA
MDA
protein assay
electrophoresis
genes knockout

Software Mentioned

SPSS
Quantity One

Related Concepts

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AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

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