Abstract
To investigate the role of PI3k/Akt signal pathway in the protective effects of propofol on intestinal and lung injury induced by intestinal ischemia/reperfusion(I/R). Male Sprague-Dawley rats were subjected to 45 min of ischemia by occluding the superior mesenteric artery and to 2h of reperfusion to establish the model of I/R. Twenty four rats were randomly divided into four groups: Sham, intestinal I/R (II/R), propofol (P), wortmannin (W). In groups P, W, propofol was injected intravenously and continuously at the onset of reperfusion via infusion pump. PI3K inhibitor (wortmannin) was administered intravenously in group W 25 min before ischemia. Intestinal tissues and lung tissues were obtained for determination of histologic injury, wet/dry weight ratio, malondialdehyde (MDA) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. Meanwhile, the expressions of caspase-3 and phosphorylated Akt (p-Akt) in intestines and lungs were detected by western blot. Propofol treatment alleviated intestinal and lung morphological changes which were observed in II/R group,Moreover, wet/dry weight ratio, the MDA level, MPO activity and expression of caspase-3 were significantly decreased whereas the SOD activity and p-Akt ...Continue Reading
References
Aug 1, 1992·Annals of Surgery·E A Deitch
Oct 1, 1970·Archives of Surgery·C J ChiuF N Gurd
Jul 6, 1995·Nature·D W NicholsonY A Lazebnik
Jan 1, 1997·Critical Care Medicine·R Barrientos-VegaA Ayensa-Rincon
Apr 6, 1999·Gut·M O OsmanS L Jensen
Oct 16, 2003·Shock·Lei ZouBruce C Kone
Feb 7, 2004·Seminars in Pediatric Surgery·Osama N El-Assal, Gail E Besner
Feb 3, 2005·The European Respiratory Journal·C S H NgA P C Yim
Aug 9, 2005·Gastroenterology·Osama N El-Assal, Gail E Besner
Aug 8, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Masahiro IizukaSumio Watanabe
Aug 16, 2006·Seminars in Respiratory and Critical Care Medicine·Fernando Frutos-VivarAndrés Esteban
Apr 24, 2007·British Journal of Pharmacology·F RoviezzoG Cirino
May 2, 2007·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Ke-Xuan LiuZhengyuan Xia
Sep 6, 2007·Drug Metabolism Reviews·Sten Orrenius
Oct 26, 2007·Anesthesia and Analgesia·Necat KaplanSavas Tezel
Oct 10, 2008·The Biochemical Journal·Vincent Duronio
Nov 21, 2008·Anesthesia and Analgesia·Ke-Xuan LiuZhengyuan Xia
Aug 26, 2009·Brain Research·Hai-yun WangYing Wang
Aug 10, 2010·American Journal of Respiratory and Critical Care Medicine·Amar KapoorChristoph Thiemermann
Sep 22, 2010·The Journal of Surgical Research·Ioanna VasileiouGeorgia Kostopanagiotou
Oct 27, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ching-Ying HuangLinda C-H Yu
Mar 15, 2011·Pediatric Surgery International·Naira BaregamianDai H Chung
Jul 28, 2011·Journal of Neurochemistry·Haiyun WangGuolin Wang
Aug 13, 2011·Basic Research in Cardiology·Sophie TamareilleFabrice Prunier
Jul 11, 2012·Shock·Shi-Hong WenKe-Xuan Liu
Nov 6, 2013·The Journal of Surgical Research·Xiang-Hu HeJia Zhan
Mar 13, 2014·Neurochemical Research·Song-sheng ShiXian-kun Tu
Jun 1, 2014·Life Sciences·Weicheng ZhaoZiqing Hei
Jun 7, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Wenshu ChenJianing Wang
Feb 11, 2015·Surgery·Chuiliang LiuYujuan Li
Citations
Jun 25, 2019·Journal of Biomolecular Structure & Dynamics·Wen-Qing JiaXian-Chao Cheng
Dec 22, 2020·Frontiers in Cell and Developmental Biology·Tsai-Chun LaiYuh-Lien Chen
Mar 6, 2021·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Yucel KaramanYalcin Guvenli
Jun 12, 2021·Journal of Cellular and Molecular Medicine·Lin XuXuekang Zhang
Jun 27, 2021·Life Sciences·Emad H M HassaneinMarwa Abdel-Raheim Ahmed
Jul 17, 2021·Molecular Medicine·Jian-Ping ZhangHua Fang
Apr 27, 2021·The Journal of Gene Medicine·Xuyang LiYubo Hu
Nov 14, 2021·Journal of Translational Medicine·Qin ZhangXue-Kang Zhang
Aug 25, 2021·Inflammation·Yubo HuJunyang Chen