The role of Polycomb-group response elements in regulation of engrailed transcription in Drosophila

Development
Sarah K DeVidoJudith A Kassis

Abstract

Polycomb group proteins are required for long-term repression of many genes in Drosophila and all metazoans. In Drosophila, DNA fragments called Polycomb-group response elements (PREs) have been identified that mediate the action of Polycomb-group proteins. Previous studies have shown that a 2 kb fragment located from -2.4 kb to -395 bp upstream of the Drosophila engrailed promoter contains a multipartite PRE that can mediate mini-white silencing and act as a PRE in an Ubx-reporter construct. Here, we study the role of this 2 kb fragment in the regulation of the engrailed gene itself. Our results show that within this 2 kb fragment, there are two subfragments that can act as PREs in embryos. In addition to their role in gene silencing, these two adjacent PRE fragments can facilitate the activation of the engrailed promoter by distant enhancers. The repressive action of the engrailed PRE can also act over a distance. A 181 bp subfragment can act as a PRE and also mediate positive effects in an enhancer-detector construct. Finally, a deletion of 530 bp of the 2 kb PRE fragment within the endogenous engrailed gene causes a loss-of-function phenotype, showing the importance of the positive regulatory effects of this PRE-containing ...Continue Reading

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Citations

Nov 17, 2010·Cellular and Molecular Life Sciences : CMLS·Vivek S Chopra
Jun 26, 2012·Molecular Genetics and Genomics : MGG·Lori A McEachern, Vett K Lloyd
Mar 15, 2012·Nucleic Acids Research·Jia ZengJianpeng Ma
Dec 2, 2009·Molecular and Cellular Biology·Melissa D CunninghamJudith A Kassis
Aug 14, 2009·Development·Deborah KwonJudith A Kassis
Jan 12, 2010·PLoS Genetics·Yuri B SchwartzVincenzo Pirrotta
Apr 24, 2014·Nature Reviews. Molecular Cell Biology·Philipp A Steffen, Leonie Ringrose
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Aug 31, 2014·Developmental Biology·Yuzhong ChengJudith A Kassis
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Feb 4, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Natalia Soshnikova
Apr 17, 2009·Mechanisms of Development·Sa Chen, Asa Rasmuson-Lestander
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Oct 19, 2017·Development·Natalie A DyeSuzanne Eaton
Oct 31, 2013·G3 : Genes - Genomes - Genetics·Jumana AlHaj AbedRichard S Jones
Jan 22, 2019·Science Advances·Sandip DeJudith A Kassis
Sep 20, 2020·Genetics·Sandip DeJudith A Kassis
May 7, 2021·Genetics·Sandip DeJudith A Kassis
Jun 4, 2021·BMC Biology·Maksim ErokhinDarya Chetverina

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