The role of polymerase chain reaction and its newer developments in feline medicine

Journal of Feline Medicine and Surgery
H LutzR Hofmann-Lehmann

Abstract

We give a brief overview on the principles of the polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), quantitative competitive PCR and real-time PCR (TaqMan technology). The literature dealing with PCR and its role in the diagnosis, pathogenesis and research of infectious diseases of the domestic cat is reviewed. Cross-contaminations which occasionally occur during handling of amplified DNA may be an important problem in the PCR laboratory. In many infectious diseases, PCR results are difficult to interpret as their predictive positive and negative values are not always known. Newer assays, such as TaqMan procedures, are becoming increasingly reliable and cost-effective. It can be expected that additional knowledge on how to interpret PCR results will soon be available.

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Citations

Jan 18, 2006·Veterinary Dermatology·Ian B SpiegelVerena K Affolter
Nov 22, 2001·Journal of Feline Medicine and Surgery·S E Andrew
Feb 4, 2010·Journal of Feline Medicine and Surgery·Claudia Hartley
Mar 1, 2002·Journal of Feline Medicine and Surgery·S Tasker, M R Lappin
Mar 29, 2002·Journal of Feline Medicine and Surgery·D Addie

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Methods Mentioned

BETA
PCR
electrophoresis
scraping
ELISA

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