The Role of PPARδ Agosnist GW501516 in Rats with Gestational Diabetes Mellitus

Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy
Jun ZhouZhijian Wang

Abstract

Gestational diabetes mellitus (GDM) is a disorder of glucose metabolism that occurs or is found for the first time during pregnancy. GDM is very harmful and urgently needs drug treatment to improve pregnancy outcome. PPARδ is involved in a variety of biological processes related to glycolipid metabolism in the body, suggesting that it may be closely related to insulin resistance and impaired glucose tolerance. The role of PPARδ agonist GW501516 in gestational diabetes has not been studied. Firstly, the rat model of GDM was established. Then, fasting blood-glucose (FGB), fasting insulin (FINS), HOMA-islet resistance index (HOMA-IR) and insulin sensitivity index (ISI) of GDM rats treated with GW501516 were measured on day 3, day 10 and day 17. Glucose tolerance test was performed on the 20th day of gestation to measure glucose tolerance in rats. The expression of PPARδ and Angptl8 in islet tissues of rats was detected by Western blot and immunohistochemistry (IHC). Histopathological changes of islet were detected by HE stain; apoptosis rate of islet cells was detected by Tunel; and expression of apoptosis-related proteins in the cells was detected by Western blot. The biochemical kits were used to detect the expression of lipid m...Continue Reading

References

Aug 23, 2000·The Journal of Clinical Investigation·J M Olefsky
Feb 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·Patrick M CatalanoJacob E Friedman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Weimin HeRonald M Evans
Aug 30, 2007·Diabetic Medicine : a Journal of the British Diabetic Association·J P H Wilding
Jul 20, 2012·Biochemical and Biophysical Research Communications·Ren Zhang
Dec 3, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiaoling WangNguan Soon Tan
Jul 30, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Bruno Martinez-PerezAna Megía
Apr 14, 2017·Scientific Reports·Marcela DoktorovaJohan W Jonker
May 4, 2017·Cell Metabolism·Weiwei FanRonald M Evans
Jan 26, 2018·Obstetrics and Gynecology·UNKNOWN Committee on Practice Bulletins—Obstetrics
Oct 31, 2018·International Journal of Molecular Sciences·Jasmine F PlowsMark H Vickers
Feb 12, 2019·European Journal of Medicinal Chemistry·Agha Zeeshan MirzaHina Shamshad

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Citations

Sep 4, 2021·Molecular Medicine Reports·Yaodong ZhaoDaimin Zhang

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Methods Mentioned

BETA
light microscopy
PCR

Software Mentioned

Image J
NIS
Spot Advance
Elements Microscope Imaging

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Apoptosis

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