PMID: 32677395Nov 1, 2018Paper

The Role of PPE25 in Mycobacterial Infections of Polymorphonuclear Neutrophils

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
You-Jun MiYue-Ying Wang

Abstract

To determine the role of PPE25 in the infection of M. smegmatis (MS) in polymorphonuclear neutrophils (PMNs). In MS-ppe25 group, PPE25 was expressed in non-pathogenic fast-growing M. tuberculosis (Mtb) that infected PMNs. The empty vector MS (MS-vec group) was served as control. Their colony formation was observed, including the size and growth curves of single colonies. The colony forming unit (CFU) indicated bacterial vitality. The percentage of lactate dehydrogenase (LDH) release measured PMN death. The role of PPE25 protein in MS infections was analyzed by reactive oxygen species (ROS) detected by flow cytometry, nitric oxide (NO) level detected by nitrate reductase, cytokine interleukin (IL) and tumor necrosis factor-α (TNF-α) detected by ELISA. PPE25 protein had no effect on MS growth, colony formation and the size of single colonies. MS-infected PMN had higher percentages of CFU and LDH release 2, 6, and 12 h after infections compared with the MS-vec group (P<0.05). MS-infected PMN also had lower levels of ROS and NO levels 2 h after infections (P<0.01), consistently higher levels of TNF-α (P<0.01), and higher levels of IL-1β infusion 6 h after MS infections (P<0.01). PPE25 protein increases the survival of MS in PMN, in...Continue Reading

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