The role of proline in osmoregulation in Phytophthora nicotianae

Fungal Genetics and Biology : FG & B
Jesmini AmbikapathyAdrienne R Hardham

Abstract

A cDNA encoding Delta(1)-pyrroline-5-carboxylate reductase (P5CR), the enzyme that catalyzes the final step in proline biosynthesis in plants and bacteria, has been cloned from the oomycete plant pathogen Phytophthora nicotianae. Genomic DNA blots indicated that P. nicotianae and P. cinnamomi each contain a single P5CR gene, whereas P. infestans contains one or two genes. Complementation of a strain of Escherichia coli defective in the P5CR protein by the P. nicotianae P5CR cDNA confirmed that the gene encoded a functional P5CR. RNA blots revealed that P5CR was expressed at a much higher level in P. nicotianae zoospores than in vegetative hyphae, sporulating hyphae, or germlings. Furthermore, P5CR mRNA levels increased with time in zoospores, demonstrating that transcription occurs in zoospores. mRNA encoding histidine and tryptophan biosynthetic enzymes was not highly and specifically expressed in zoospores, indicating that the developmental pattern of P5CR expression was not simply a reflection of overall amino acid biosynthesis as might be required for protein synthesis. Measurement of free proline in P. nicotianae at different stages of the life cycle revealed that proline concentration was highest in sporulating hyphae. Ve...Continue Reading

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Citations

Dec 4, 2003·Molecular Genetics and Genomics : MGG·D SkalameraA R Hardham
Oct 15, 2013·Applied Biochemistry and Biotechnology·S Nishanth Kumar, Bala Nambisan
Dec 21, 2004·Nature Reviews. Microbiology·Howard S Judelson, Flavio A Blanco
Nov 29, 2002·Eukaryotic Cell·Howard S Judelson, Samuel Roberts
Nov 1, 2005·Molecular Plant Pathology·Adrienne R Hardham
Nov 4, 2006·Cellular Microbiology·Adrienne R Hardham
Feb 15, 2005·Fungal Genetics and Biology : FG & B·Laura J Grenville-BriggsPieter van West
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Roger R LewAshley Garrill

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