PMID: 7520224Jan 1, 1994Paper

The role of proteases in stratum corneum: involvement in stratum corneum desquamation

Archives of Dermatological Research
Y SuzukiI Horii

Abstract

The effects of protease inhibitors on cell dissociation were studied in vitro in order to examine the involvement of proteases in stratum corneum desquamation. Stratum corneum sheet (peeled from human backs after sunburn) was incubated in a detergent mixture containing 8 mM N,N-dimethyldodecylamine oxide, 2 mM sodium lauryl sulphate and 60 micrograms/ml kanamycin with or without protease inhibitors, and the number of released cells was counted after incubation for 48 h. Cell dissociation was inhibited strongly by antipain or aprotinin, but not at all by N-[N-(L-3-transcarboxyoxiran-2-carbonyl)-L-leucyl]-agmatin, N-ethylmaleimide or pepstatin, which suggests that only serine proteases are associated with desquamation. Furthermore, leupeptin and chymostatin each reduced cell dissociation about half as effectively as aprotinin or antipain, while a mixture of leupeptin and chymostatin prevented stratum corneum dissociation as potently as antipain or aprotinin. In addition, the activity of chymotrypsin-like protease in scaly skin was higher than that in normal skin, as we have previously found for trypsin-like protease. These results suggest that both trypsin-like and chymotrypsin-like serine proteases are involved in stratum corneu...Continue Reading

References

Oct 1, 1990·The Journal of Investigative Dermatology·T Egelrud, A Lundström
Jan 1, 1991·Archives of Dermatological Research·T Egelrud, A Lundström
Jan 1, 1990·Archives of Dermatological Research·S J Chapman, A Walsh
Sep 1, 1988·Cell and Tissue Research·M M BryskE Barlow
Jan 1, 1989·Experimental Cell Biology·M M BryskT Bell
Oct 1, 1988·The Journal of Investigative Dermatology·A Lundström, T Egelrud
Jan 1, 1987·Archives of Dermatological Research·D L BissettL F Patrick
Apr 1, 1967·Archives of Dermatology·H Baker, A M Kligman
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N HorieW L Epstein
Dec 1, 1981·Archives of Dermatology·E H EpsteinP M Elias
Jun 1, 1983·The Journal of Investigative Dermatology·P M Elias

❮ Previous
Next ❯

Citations

Jun 21, 2002·Biopolymers·E Michael AttasShuliang L Zhang
May 1, 1997·Medical & Biological Engineering & Computing·O G MartinsenE S Kongshaug
Oct 12, 2012·Cell and Tissue Research·Anthony V Rawlings, Rainer Voegeli
Dec 29, 1998·Journal of Dermatological Science·J SatoJ Koyama
Jan 5, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Dieter SwatschekJörg Kreuter
Nov 3, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·C BaumL G Fleischer
Jun 27, 2002·Contact Dermatitis·Gianluigi GiannelliGiovanni Angelini
May 16, 2000·The British Journal of Dermatology·M Denda, T Tsuchiya
Feb 24, 2005·Contact Dermatitis·Ke-Jen YuHsin-Chun Ho
Jun 1, 1997·Experimental Dermatology·P FilipeA Fernandes
Jun 10, 2010·Photochemistry and Photobiology·Anup Sharma
Sep 29, 2004·Pharmacology·Izabela Buraczewska, Marie Lodén
Nov 14, 2013·PloS One·Zoltán NoszticziusJános Szegedi
Oct 24, 2003·American Journal of Clinical Dermatology·Marie Lodén
Jul 5, 2011·Annual Review of Cell and Developmental Biology·Roman Szabo, Thomas H Bugge
Aug 12, 1998·The Journal of Investigative Dermatology·J SatoJ Koyama
Jun 16, 2005·The Journal of Investigative Dermatology·Anthony V Rawlings, Paul J Matts
Nov 20, 2009·The Journal of Investigative Dermatology·Jotaro NakanishiToshihiko Hibino
Jun 6, 2009·The Journal of Investigative Dermatology·Michael J CorkSimon J Ward
Nov 15, 2013·The Journal of Investigative Dermatology·Wei XuSeok J Hong
Jan 5, 2000·The Journal of Investigative Dermatology·I E EkholmT Egelrud
Nov 5, 2005·Journal of the European Academy of Dermatology and Venereology : JEADV·M Lodén
Mar 18, 2008·The British Journal of Dermatology·K KikuchiUNKNOWN Japanese Cosmetic Scientist Task Force for Skin Care of Atopic Dermatitis
May 21, 2008·International Journal of Cosmetic Science·Q C HeT Polefka
Jun 14, 2016·Indian Journal of Dermatology·Anisha SethiM L Gambhir
May 13, 2014·The British Journal of Dermatology·B SinghC R Harding
May 6, 2003·Journal of Lipid Research·Charles A Strott, Yuko Higashi
Aug 16, 2005·The Journal of Investigative Dermatology·Daniel RedoulésNancy De Viguerie
May 1, 1997·Microscopy Research and Technique·M Fartasch
Jul 12, 2018·Journal of Wound, Ostomy, and Continence Nursing : Official Publication of the Wound, Ostomy and Continence Nurses Society·Sabrina S FalloonAlan Cottenden
May 22, 2008·International Journal of Cosmetic Science·A G SchepkyS S Biel
Dec 23, 2008·International Journal of Cosmetic Science·R VoegeliT Schreier
May 23, 2008·International Journal of Cosmetic Science·A V Rawlings
Nov 1, 1995·Bio/technology·M R PrausnitzJ C Weaver
May 3, 2019·Acta Crystallographica. Section F, Structural Biology Communications·James H ThorpeAlain Hovnanian
Jul 9, 1998·Oral Diseases·A T CruchleyJ S Greenspan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.