PMID: 10735618Mar 29, 2000

The role of protein kinase A in anaerobic energy production during liver storage

Journal of Hepatology
T A ChurchillN M Kneteman

Abstract

During cold liver storage in University of Wisconsin solution, glycolysis is inhibited by declining intracellular pH and a reduction in glycogen phosphorylase activity. The current study investigated the effects of a histidine-buffered, modified University of Wisconsin solution with cyclic-AMP analogue plus phosphodiesterase inhibitors to optimize both pH and PK A-mediated limits on glycolytic energy production. In an isolated rodent-liver system, dioctanoyl-cAMP was supplemented with each phosphodiesterase inhibitor (isobutylmethylxanthine, papaverine, Ro 20-1724, dipyridamole). Once the most efficacious combination was determined, a separate group of livers was cold-stored for 24 h and then reperfused at 37 degrees C to examine regeneration of high energy adenylates. Lactate accumulation in the histidine-lactobionate-raffinose group was 8.7 micromol/g; net increases were greater with all four phosphodiesterase inhibitors with dioctanoyl-cAMP; dipyridamole resulted in a maximum increase of 16.7 micromol/g. ATP was consistently higher in all treatment groups with phosphodiesterase inhibitors throughout 24 h; even after 10-24 h, levels with dipyridamole-treatment were 250-280% higher than with University of Wisconsin (p<0.05). A...Continue Reading

References

Jan 1, 1995·Transplant International : Official Journal of the European Society for Organ Transplantation·T A ChurchillB J Fuller
Jun 20, 1995·Archives of Biochemistry and Biophysics·T A ChurchillB J Fuller
Jan 1, 1994·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T A ChurchillB J Fuller
Apr 15, 1996·Archives of Biochemistry and Biophysics·N ErcanF Q Nuttall

Citations

May 12, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M Kukan, P Haddad
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R P PulisT A Churchill

Related Concepts

N(6),O(2)-dioctanoyl cyclic AMP
Adenine Nucleotides
Cyclic AMP, (R)-Isomer
Anaerobiosis
Drug Combinations
Energy Metabolism
Phosphorylases
Liver
Liver Circulation
Phosphodiesterase Inhibitors

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